Journal article
Exploring the associations between microRNA expression profiles and environmental pollutants in human placenta from the National Children's Study (NCS)
Epigenetics, Vol.10(9), pp.793-802
09/02/2015
DOI: 10.1080/15592294.2015.1066960
PMCID: PMC4622837
PMID: 26252056
Abstract
The placenta is the principal regulator of the in utero environment, and disruptions to this environment can result in adverse offspring health outcomes. To better characterize the impact of in utero perturbations, we assessed the influence of known environmental pollutants on the expression of microRNA (miRNA) in placental samples collected from the National Children's Study (NCS) Vanguard birth cohort. This study analyzed the expression of 654 miRNAs in 110 term placentas. Environmental pollutants measured in these placentas included dichlorodiphenyldichloroethylene (DDE), bisphenol A (BPA), polybrominated diphenyl ethers (PBDEs), polychlorinated biphenyls (PCBs), arsenic (As), mercury (Hg), lead (Pb), and cadmium (Cd). A moderated t-test was used to identify a panel of differentially expressed miRNAs, which were further analyzed using generalized linear models. We observed 112 miRNAs consistently expressed in >70% of the samples. Consistent with the literature, miRNAs located within the imprinted placenta-specific C19MC cluster, specifically mir-517a, mir-517c, mir-522, and mir-23a, are among the top expressed miRNA in our study. We observed a positive association between PBDE 209 and miR-188-5p and an inverse association between PBDE 99 and let-7c. Both PCBs and Cd were positively associated with miR-1537 expression level. In addition, multiple let-7 family members were downregulated with increasing levels of Hg and Pb. We did not observe DDE or BPA levels to be associated with placental miRNA expression. This is the first birth cohort study linking environmental pollutants and placental expression of miRNAs. Our results suggest that placental miRNA profiles may signal in utero exposures to environmental chemicals.
Details
- Title: Subtitle
- Exploring the associations between microRNA expression profiles and environmental pollutants in human placenta from the National Children's Study (NCS)
- Creators
- Qian Li - Departments of Preventive Medicine, Pediatrics, Oncological Science, Obstetrics, Gynecology and Reproductive Sciences; Icahn School of Medicine at Mount SinaiMaya A Kappil - Departments of Preventive Medicine, Pediatrics, Oncological Science, Obstetrics, Gynecology and Reproductive Sciences; Icahn School of Medicine at Mount SinaiAn Li - School of Public Health; University of Illinois at ChicagoPriyanthi S Dassanayake - School of Public Health; University of Illinois at ChicagoThomas H Darrah - School of Earth Sciences; Ohio State UniversityAlan E Friedman - Departments of Obs/Gyn and Environmental Medicine; University of Rochester School of Medicine and DentistryMichelle Friedman - Departments of Obs/Gyn and Environmental Medicine; University of Rochester School of Medicine and DentistryLuca Lambertini - Departments of Preventive Medicine, Pediatrics, Oncological Science, Obstetrics, Gynecology and Reproductive Sciences; Icahn School of Medicine at Mount SinaiPhilip Landrigan - Departments of Preventive Medicine, Pediatrics, Oncological Science, Obstetrics, Gynecology and Reproductive Sciences; Icahn School of Medicine at Mount SinaiChristopher J Stodgell - Departments of Obs/Gyn and Environmental Medicine; University of Rochester School of Medicine and DentistryYulin Xia - School of Public Health; University of Illinois at ChicagoJessica A Nanes - School of Public Health; University of Illinois at ChicagoKjersti M Aagaard - Department of Obs/Gyn; Baylor School of MedicineEric E Schadt - Department of Genetics and Genomic Sciences and Multiscale Biology; Icahn School of Medicine at Mt. SinaiJeff C Murray - Department of Genetics; University of IowaEdward B Clark - Department of Pediatrics and Obs/Gyn; University of UtahNancy Dole - Carolina Population Center; University of North CarolinaJennifer Culhane - The Children's Hospital of PhiladelphiaJames Swanson - Department of Pediatrics; University of California at IrvineMichael Varner - Department of Pediatrics and Obs/Gyn; University of UtahJack Moye - Eunice Kennedy Shriver National Institute of Child Health and Human Development; National Institutes of HealthCarol Kasten - Division of Pediatric and Maternal Health; US Food and Drug AdministrationRichard K Miller - Departments of Obs/Gyn and Environmental Medicine; University of Rochester School of Medicine and DentistryJia Chen - Departments of Preventive Medicine, Pediatrics, Oncological Science, Obstetrics, Gynecology and Reproductive Sciences; Icahn School of Medicine at Mount Sinai
- Resource Type
- Journal article
- Publication Details
- Epigenetics, Vol.10(9), pp.793-802
- DOI
- 10.1080/15592294.2015.1066960
- PMID
- 26252056
- PMCID
- PMC4622837
- NLM abbreviation
- Epigenetics
- ISSN
- 1559-2294
- eISSN
- 1559-2308
- Publisher
- Taylor & Francis
- Language
- English
- Date published
- 09/02/2015
- Academic Unit
- Anatomy and Cell Biology; Stead Family Department of Pediatrics; Epidemiology; Pediatric Dentistry; Craniofacial Anomalies Research Center; Dental Research
- Record Identifier
- 9984025357802771
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