Journal article
Exploring the genomic basis of early childhood caries: a pilot study
International journal of paediatric dentistry, Vol.28(2), pp.217-225
03/2018
DOI: 10.1111/ipd.12344
PMCID: PMC5811369
PMID: 29057527
Abstract
A genetic component in early childhood caries (ECC) is theorized, but no genome-wide investigations of ECC have been conducted. This pilot study is part of a long-term research program aimed to: (1) determine the proportion of ECC variance attributable to the human genome and (2) identify ECC-associated genetic loci. The study's community-based sample comprised 212 children (mean age=39 months; range = 30-52 months; males = 55%; Hispanic/Latino = 35%, African-American = 32%; American Academy of Pediatric Dentistry definition of ECC prevalence = 38%). Approximately 2.4 million single nucleotide polymorphisms (SNPs) were genotyped using DNA purified from saliva. A P < 5 × 10 criterion was used for genome-wide significance. SNPs with P < 5 × 10 were followed-up in three independent cohorts of 921 preschool-age children with similar ECC prevalence. SNPs with minor allele frequency ≥5% explained 52% (standard error = 54%) of ECC variance (one-sided P = 0.03). Unsurprisingly, given the pilot's small sample size, no genome-wide significant associations were found. An intergenic locus on 4q32 (rs4690994) displayed the strongest association with ECC [P = 2.3 × 10 ; odds ratio (OR) = 3.5; 95% confidence interval (CI) = 2.1-5.9]. Thirteen loci with suggestive associations were followed-up - none showed evidence of association in the replication samples. This study's findings support a heritable component of ECC and demonstrate the feasibility of conducting genomics studies among preschool-age children.
Details
- Title: Subtitle
- Exploring the genomic basis of early childhood caries: a pilot study
- Creators
- Jami L Ballantine - Department of Pediatric Dentistry, School of Dentistry, University of North Carolina-Chapel Hill, Chapel Hill, NC, USAJenna C Carlson - Department of Human Genetics, Graduate School of Public Health, University of Pittsburgh, Pittsburgh, PA, USAAndrea G Ferreira Zandoná - Department of Operative Dentistry, School of Dentistry, University of North Carolina-Chapel Hill, Chapel Hill, NC, USACary Agler - Oral and Craniofacial Health Sciences, School of Dentistry, University of North Carolina-Chapel Hill, Chapel Hill, NC, USALeslie P Zeldin - Oral and Craniofacial Health Sciences, School of Dentistry, University of North Carolina-Chapel Hill, Chapel Hill, NC, USARichard Gary Rozier - Department of Health Policy and Management, Gillings School of Global Public Health, University of North Carolina-Chapel Hill, Chapel Hill, NC, USAMichael W Roberts - Department of Pediatric Dentistry, School of Dentistry, University of North Carolina-Chapel Hill, Chapel Hill, NC, USAPatricia V Basta - Biospecimen Processing Facility core, University of North Carolina, Chapel Hill, NC, USAJason Luo - Mammalian Genotyping core, University of North Carolina, Chapel Hill, NC, USAMikafui E Antonio-Obese - Oral and Craniofacial Health Sciences, School of Dentistry, University of North Carolina-Chapel Hill, Chapel Hill, NC, USADaniel W McNeil - Departments of Dental Practice & Rural Health and Psychology, West Virginia University, Morgantown, WV, USARobert J Weyant - Department of Dental Public Health and Information Management, School of Dental Medicine, University of Pittsburgh, Pittsburgh, PA, USARichard J Crout - Department of Periodontics, School of Dentistry, West Virginia University, Morgantown, WV, USARebecca L Slayton - Department of Pediatric Dentistry, School of Dentistry, University of Washington, Seattle, WA, USASteven M Levy - Department of Epidemiology, University of Iowa College of Public Health, Iowa City, IA, USAJohn R Shaffer - Department of Oral Biology, School of Dental Medicine, University of Pittsburgh, Pittsburgh, PA, USAMary L Marazita - Clinical and Translational Science Institute, School of Medicine, University of Pittsburgh, Pittsburgh, PA, USAKari E North - Department of Epidemiology, Gillings School of Global Public Health, University of North Carolina-Chapel Hill, Chapel Hill, NC, USAKimon Divaris - Department of Epidemiology, Gillings School of Global Public Health, University of North Carolina-Chapel Hill, Chapel Hill, NC, USA
- Resource Type
- Journal article
- Publication Details
- International journal of paediatric dentistry, Vol.28(2), pp.217-225
- DOI
- 10.1111/ipd.12344
- PMID
- 29057527
- PMCID
- PMC5811369
- NLM abbreviation
- Int J Paediatr Dent
- ISSN
- 0960-7439
- eISSN
- 1365-263X
- Publisher
- England
- Grant note
- R01 DE014899 / NIDCR NIH HHS R03 DE024264 / NIDCR NIH HHS U01 DE018903 / NIDCR NIH HHS P30 ES010126 / NIEHS NIH HHS R01 DE009551 / NIDCR NIH HHS U01 DE025046 / NIDCR NIH HHS R01 DE012101 / NIDCR NIH HHS R01 DE018236 / NIDCR NIH HHS
- Language
- English
- Date published
- 03/2018
- Academic Unit
- Preventive and Community Dentistry; Epidemiology; Pediatric Dentistry
- Record Identifier
- 9983917689302771
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