Exponential increase in neutralizing and spike specific antibodies following vaccination of COVID-19 convalescent plasma donors

Molly A Vickers; Alan Sariol; Judith Leon; Alexandra Ehlers; Aaron V Locher; Kerry A Dubay; Laura Collins; Dena Voss; Abby E Odle; Myrl Holida; Anna E Merrill; Stanley Perlman; C Michael Knudson

doi:10.1111/trf.16401

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Exponential increase in neutralizing and spike specific antibodies following vaccination of COVID-19 convalescent plasma donors

Journal article

Open access

Peer reviewed

Exponential increase in neutralizing and spike specific antibodies following vaccination of COVID-19 convalescent plasma donors

Molly A Vickers, Alan Sariol, Judith Leon, Alexandra Ehlers, Aaron V Locher, Kerry A Dubay, Laura Collins, Dena Voss, Abby E Odle, Myrl Holida, …

Transfusion (Philadelphia, Pa.), Vol.61(7), pp.2099-2106

04/08/2021

DOI: 10.1111/trf.16401

PMID: 33829513

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Abstract

With the recent approval of COVID-19 vaccines, recovered COVID-19 subjects who are vaccinated may be ideal candidates to donate COVID-19 convalescent plasma (CCP). Eleven recovered COVID-19 patients were screened to donate CCP. All had molecularly confirmed COVID-19, and all but one were antibody positive by chemiluminescence immunoassay (DiaSorin) prior to vaccination. All were tested again for antibodies 11-21 days after they were vaccinated (Pfizer/Moderna). All showed dramatic increases (~50-fold) in spike-specific antibody levels and had at least a 20-fold increase in the IC50 neutralizing antibody titer based on plaque reduction neutralization testing (PRNT). The spike-specific antibody levels following vaccination were significantly higher than those seen in any non-vaccinated COVID-19 subjects tested to date at our facility. Spike-specific and neutralizing antibodies demonstrated dramatic increases following a single vaccination after COVID-19 infection, which significantly exceeded values seen with COVID-19 infection alone. Recovered COVID-19 subjects who are vaccinated may make ideal candidates for CCP donation.

immunology (other than RBC serology)

transfusion Practices (Adult)

Details

Title: Subtitle: Exponential increase in neutralizing and spike specific antibodies following vaccination of COVID-19 convalescent plasma donors
Creators: Molly A Vickers - Department of Microbiology and Immunology, University of Iowa, Iowa City, Iowa
Alan Sariol - Department of Microbiology and Immunology, University of Iowa, Iowa City, Iowa
Judith Leon - Department of Pathology, University of Iowa, Iowa City, Iowa
Alexandra Ehlers - Department of Pathology, University of Iowa, Iowa City, Iowa
Aaron V Locher - Department of Pathology, University of Iowa, Iowa City, Iowa
Kerry A Dubay - Department of Pathology, University of Iowa, Iowa City, Iowa
Laura Collins - Department of Pathology, University of Iowa, Iowa City, Iowa
Dena Voss - Department of Pathology, University of Iowa, Iowa City, Iowa
Abby E Odle - Department of Microbiology and Immunology, University of Iowa, Iowa City, Iowa
Myrl Holida - Department of Pediatrics, University of Iowa, Iowa City, Iowa
Anna E Merrill - Department of Pathology, University of Iowa, Iowa City, Iowa
Stanley Perlman - Department of Pediatrics, University of Iowa, Iowa City, Iowa
C Michael Knudson - Department of Pathology, University of Iowa, Iowa City, Iowa
Resource Type: Journal article
Publication Details: Transfusion (Philadelphia, Pa.), Vol.61(7), pp.2099-2106
DOI: 10.1111/trf.16401
PMID: 33829513
NLM abbreviation: Transfusion
eISSN: 1537-2995
Publisher: United States
Language: English
Date published: 04/08/2021
Academic Unit: Microbiology and Immunology; Stead Family Department of Pediatrics; Pathology; Iowa Neuroscience Institute; Radiation Oncology; Injury Prevention Research Center; Infectious Disease (Pediatrics)
Record Identifier: 9984070335702771

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