Journal article
Exposure to structurally unique β‐d‐glucans differentially affects inflammatory responses in male mouse lungs
Physiological reports, Vol.12(12), e16115
06/2024
DOI: 10.14814/phy2.16115
PMCID: PMC11194181
PMID: 38923221
Appears in UI Libraries Support Open Access
Abstract
Pro-inflammatory fungal β-d-glucan (BDG) polysaccharides cause respiratory pathology. However, specific immunological effects of unique BDG structures on pulmonary inflammation are understudied. We characterized the effect of four unique fungal BDGs with unique branching patterns, solubility, and molecular weights in murine airways. Scleroglucan (1 → 3)(1 → 6)-highly branched BDG, laminarin (1 → 3)(1 → 6)-branched BDG, curdlan (1 → 3)-linear BDG, and pustulan (1 → 6)-linear BDG were assessed by nuclear magnetic resonance spectroscopy. Each BDG was tested by inhalation model with C3HeB/FeJ mice and compared to saline-exposed control mice and unexposed sentinels (n = 3–19). Studies were performed ±heat-inactivation (1 h autoclave) to increase BDG solubility. Outcomes included bronchoalveolar lavage (BAL) differential cell counts (macrophages, neutrophils, lymphocytes, eosinophils), cytokines, serum IgE, and IgG2a (multiplex and ELISA). Ex vivo primary cells removed from lungs and plated at monolayer were stimulated (BDG, lipopolysaccharide (LPS), anti-CD3), and cytokines compared to unstimulated cells. Right lung histology was performed. Inhalation of BDGs with distinct branching patterns exhibited varying inflammatory potency and immunogenicity. Lichen-derived (1 → 6)-linear pustulan was the most pro-inflammatory BDG, increasing inflammatory infiltrate (BAL), serum IgE and IgG2a, and cytokine production. Primed lung cells responded to secondary LPS stimulation with a T-cell-specific response to pustulan. Glucan source and solubility should be considered in exposure and toxicological studies.
Details
- Title: Subtitle
- Exposure to structurally unique β‐d‐glucans differentially affects inflammatory responses in male mouse lungs
- Creators
- Nervana Metwali - University of Iowa, Occupational and Environmental HealthEmma M. Stapleton - University of IowaSuzana Hadina - University of IowaPeter S. Thorne - University of Iowa
- Resource Type
- Journal article
- Publication Details
- Physiological reports, Vol.12(12), e16115
- Publisher
- Wiley
- DOI
- 10.14814/phy2.16115
- PMID
- 38923221
- PMCID
- PMC11194181
- ISSN
- 2051-817X
- eISSN
- 2051-817X
- Alternative title
- Metwali et al
- Language
- English
- Date published
- 06/2024
- Academic Unit
- Civil and Environmental Engineering; Occupational and Environmental Health; Internal Medicine
- Record Identifier
- 9984649048402771
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