Expression Profiling of Transcription Factors in B-or T-Acute Lymphoblastic Leukemia/Lymphoma and Burkitt Lymphoma: Usefulness of PAX5 Immunostaining as Pan-Pre-B-Cell Marker

Michel R NASR; Nancy ROSENTHAL; Sergei SYRBU

doi:10.1309/AJCPYP00JNUFWCCY

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Expression Profiling of Transcription Factors in B-or T-Acute Lymphoblastic Leukemia/Lymphoma and Burkitt Lymphoma: Usefulness of PAX5 Immunostaining as Pan-Pre-B-Cell Marker

Journal article

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Peer reviewed

Expression Profiling of Transcription Factors in B-or T-Acute Lymphoblastic Leukemia/Lymphoma and Burkitt Lymphoma: Usefulness of PAX5 Immunostaining as Pan-Pre-B-Cell Marker

Michel R NASR, Nancy ROSENTHAL and Sergei SYRBU

American journal of clinical pathology, Vol.133(1), pp.41-48

2010

DOI: 10.1309/AJCPYP00JNUFWCCY

PMID: 20023257

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Abstract

The optimal use of transcription factors to determine B-lineage specificity in B-acute lymphoblastic leukemia/lymphoma (B-ALL) has not been fully investigated. We undertook an extensive immunohistochemical study of a panel of B-cell transcription factors in B- and T-ALL and Burkitt lymphoma to evaluate those with the best specificity and sensitivity. Tissue microarrays were constructed from 34 B-ALL, 19 T-ALL, and 30 Burkitt lymphoma samples. All 34 (100%) cases of B-ALL expressed PAX5; 32 (94%), BOB.1; 33 (97%), PU.1; 29 (85%), CD79a; 27 (79%), CD22; 2 (6%), CD20; 9 (26%), OCT-2; and 3 (9%), MUM1. Burkitt lymphoma cases were positive for PAX5 (30/30 [100%]), BOB.1 (27/30 [90%]), PU.1 (23/30 [77%]), CD79a (29/30 [97%]), CD22 (14/30 [47%]), CD20 (30/30 [100%]), OCT-2 (23/30 [77%]), and MUM1 (5/30 [17%]). T-ALLs were only positive for PU.1 (15/19 [79%]) and BOB.1 (12/19 [63%]). PAX5 demonstrated better specificity for B-lineage determination than BOB.1 and PU.1 and better sensitivity than CD79a, CD22, and CD20. These findings suggest that PAX5 has the greatest diagnostic usefulness and lineage determination in B-ALL, especially in cases with an inadequate specimen for flow cytometric analysis.

Infectious Diseases

Hematologic and hematopoietic diseases

Investigative techniques, diagnostic techniques (general aspects)

Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques

Viral diseases

Leukemias. Malignant lymphomas. Malignant reticulosis. Myelofibrosis

Biological and medical sciences

Medical sciences

Details

Title: Subtitle: Expression Profiling of Transcription Factors in B-or T-Acute Lymphoblastic Leukemia/Lymphoma and Burkitt Lymphoma: Usefulness of PAX5 Immunostaining as Pan-Pre-B-Cell Marker
Creators: Michel R NASR - Department of Pathology, University of Iowa Hospitals and Clinics, Iowa City, United States
Nancy ROSENTHAL - Department of Pathology, University of Iowa Hospitals and Clinics, Iowa City, United States
Sergei SYRBU - Department of Pathology, University of Iowa Hospitals and Clinics, Iowa City, United States
Resource Type: Journal article
Publication Details: American journal of clinical pathology, Vol.133(1), pp.41-48
Publisher: American Society of Clinical Pathologists; Chicago, IL
DOI: 10.1309/AJCPYP00JNUFWCCY
PMID: 20023257
ISSN: 0002-9173
eISSN: 1943-7722
Language: English
Date published: 2010
Academic Unit: Pathology
Record Identifier: 9984047881702771

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