Journal article
Expression and Fine Mapping of Murine Vasoactive Intestinal Peptide Receptor 1
Journal of molecular neuroscience, Vol.17(3), pp.311-324
2001
DOI: 10.1385/JMN:17:3:311
PMID: 11859927
Abstract
Vasoactive intestinal peptide (VIP) plays multiple roles in the nervous, endocrine, and immune systems as a neurotransmitter, a hormone, and a cytokine. VIP is widely distributed in neurons of the central and peripheral nervous systems (CNS/PNS), and recently has been found to be an important neuroprotective agent. VIP actions are mediated through specific G protein-coupled receptors. We have cloned the cDNA of VIP receptor subtype 1 (VIPR1+ or VPAC1) and have demonstrated the quantitative expression profile in mice. Fluorometric real-time reverse transcription-polymerase chain reaction (RT-PCR) analysis demonstrated that VPAC1 is expressed in all tissues examined. Expression was highest in the small intestine and colon followed by the liver and brain. The high level of VPAC1 expression in forebrain and cerebellum suggests that VPAC1 may mediate the neuroprotective effect of VIP. We have refined the chromosomal localization of the mouse, rat, and human VPAC1 genes. This fine mapping of the VPAC1 gene extends the respective regions of synteny between the distal region of mouse chromosome 9, rat chromosome 8q32, and human chromosome 3p21.33-p21.31. Thus, VPAC1 constitutes a functional-positional candidate for the tumor-suppressor function mapped to human 3p22-p21 where loss-of-heterozygosity is observed in small-cell lung carcinoma (SCLC) cell lines and primary tumors. Availability of the cDNA sequences for mouse VPAC1 will facilitate the generation of VPAC1 null mutant animals. Such studies will ultimately enhance our understanding of the role of VIP in the nervous system.
Details
- Title: Subtitle
- Expression and Fine Mapping of Murine Vasoactive Intestinal Peptide Receptor 1
- Creators
- Bahri KaracaySue O'DorisioKim KasiwClay HollenbackRalf Krahe
- Resource Type
- Journal article
- Publication Details
- Journal of molecular neuroscience, Vol.17(3), pp.311-324
- DOI
- 10.1385/JMN:17:3:311
- PMID
- 11859927
- ISSN
- 0895-8696
- eISSN
- 1559-1166
- Language
- English
- Date published
- 2001
- Academic Unit
- Stead Family Department of Pediatrics; Iowa Neuroscience Institute
- Record Identifier
- 9984065734802771
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