Journal article
Expression and Subunit Interaction of Voltage-Dependent Ca2+ Channels in PC12 Cells
The Journal of neuroscience, Vol.16(23), pp.7557-7565
12/01/1996
DOI: 10.1523/JNEUROSCI.16-23-07557.1996
PMID: 8922412
Abstract
Nerve growth factor (NGF)-induced differentiation in PC12 cells is accompanied by changes in the expression of voltage-dependent Ca
2+
channels. Ca
2+
channels are multimeric complexes composed of at least three subunits (α
1
, β, and α
2
δ) and are involved in neuronal migration, gene expression, and neurotransmitter release. Although attempts have been undertaken to elucidate NGF regulation of Ca
2+
channel expression, the changes in subunit composition of these channels during differentiation still remain uncertain. In the present study, patch-clamp recordings show that in addition to the previously documented L-type and N-type Ca
2+
currents, undifferentiated PC12 cells also express an ω-agatoxin-IVA-sensitive (P/Q-type) component. In addition, the corresponding mRNA encoding the pore-forming α
1
subunits for these channels (C, B, and A, respectively) was detected. Likewise, mRNA for three distinct auxiliary β subunits (1, 2, 3) were also found, β
3
protein being dominantly expressed. Immunoprecipitation experiments show that the N-type Ca
2+
channel is associated with either a β
2
or β
3
subunit and that NGF increases the channel expression without affecting its β subunit association. These results (1) indicate that the diversity of Ca
2+
currents in PC12 cells arise from the expression of three distinct α
1
and three different β subunit genes; (2) support a model for heterogenous β subunit association of the N-type Ca
2+
channel in a single cell type; and (3) suggest that the regulation of the N-type Ca
2+
channel during NGF-mediated differentiation involves an increase in the number of functional channels with no apparent changes in subunit composition.
Details
- Title: Subtitle
- Expression and Subunit Interaction of Voltage-Dependent Ca2+ Channels in PC12 Cells
- Creators
- Hongyan LiuRicardo FelixChristina A GurnettMichel De WaardDerrick R WitcherKevin P Campbell
- Resource Type
- Journal article
- Publication Details
- The Journal of neuroscience, Vol.16(23), pp.7557-7565
- DOI
- 10.1523/JNEUROSCI.16-23-07557.1996
- PMID
- 8922412
- NLM abbreviation
- J Neurosci
- ISSN
- 0270-6474
- eISSN
- 1529-2401
- Publisher
- Society for Neuroscience
- Language
- English
- Date published
- 12/01/1996
- Academic Unit
- Neurology; Molecular Physiology and Biophysics; Iowa Neuroscience Institute
- Record Identifier
- 9984068372402771
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