Expression and activity of acid-sensing ion channels in the mouse anterior pituitary

Jianyang Du; Leah R Reznikov; Michael J Welsh

doi:10.1371/journal.pone.0115310

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Expression and activity of acid-sensing ion channels in the mouse anterior pituitary

Journal article

Open access

Peer reviewed

Expression and activity of acid-sensing ion channels in the mouse anterior pituitary

Jianyang Du, Leah R Reznikov and Michael J Welsh

PloS one, Vol.9(12), pp.e115310-e115310

2014

DOI: 10.1371/journal.pone.0115310

PMCID: PMC4266673

PMID: 25506946

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Abstract

Acid sensing ion channels (ASICs) are proton-gated cation channels that are expressed in the nervous system and play an important role in fear learning and memory. The function of ASICs in the pituitary, an endocrine gland that contributes to emotions, is unknown. We sought to investigate which ASIC subunits were present in the pituitary and found mRNA expression for all ASIC isoforms, including ASIC1a, ASIC1b, ASIC2a, ASIC2b, ASIC3 and ASIC4. We also observed acid-evoked ASIC-like currents in isolated anterior pituitary cells that were absent in mice lacking ASIC1a. The biophysical properties and the responses to PcTx1, amiloride, Ca2+ and Zn2+ suggested that ASIC currents were mediated predominantly by heteromultimeric channels that contained ASIC1a and ASIC2a or ASIC2b. ASIC currents were also sensitive to FMRFamide (Phe-Met-Arg-Phe amide), suggesting that FMRFamide-like compounds might endogenously regulate pituitary ASICs. To determine whether ASICs might regulate pituitary cell function, we applied low pH and found that it increased the intracellular Ca2+ concentration. These data suggest that ASIC channels are present and functionally active in anterior pituitary cells and may therefore influence their function.

Gene Expression

Calcium - metabolism

Acid Sensing Ion Channel Blockers - pharmacology

RNA, Messenger - metabolism

Pituitary Gland, Anterior - metabolism

Animals

Acid Sensing Ion Channels - physiology

Pituitary Gland, Anterior - physiology

Pituitary Gland, Anterior - drug effects

Acid Sensing Ion Channels - genetics

Mice

FMRFamide - pharmacology

Acid Sensing Ion Channels - metabolism

Details

Title: Subtitle: Expression and activity of acid-sensing ion channels in the mouse anterior pituitary
Creators: Jianyang Du - Howard Hughes Medical Institute, Roy J and Lucille A Carver College of Medicine, University of Iowa, Iowa City, Iowa, United States of America; Department of Internal Medicine, Roy J and Lucille A Carver College of Medicine, University of Iowa, Iowa City, Iowa, United States of America
Leah R Reznikov - Department of Internal Medicine, Roy J and Lucille A Carver College of Medicine, University of Iowa, Iowa City, Iowa, United States of America
Michael J Welsh - Howard Hughes Medical Institute, Roy J and Lucille A Carver College of Medicine, University of Iowa, Iowa City, Iowa, United States of America; Department of Internal Medicine, Roy J and Lucille A Carver College of Medicine, University of Iowa, Iowa City, Iowa, United States of America; Department of Molecular Physiology and Biophysics, Roy J and Lucille A Carver College of Medicine, University of Iowa, Iowa City, Iowa, United States of America
Resource Type: Journal article
Publication Details: PloS one, Vol.9(12), pp.e115310-e115310
DOI: 10.1371/journal.pone.0115310
PMID: 25506946
PMCID: PMC4266673
NLM abbreviation: PLoS One
ISSN: 1932-6203
eISSN: 1932-6203
Publisher: Public Library of Science; United States
Grant note: Howard Hughes Medical Institute K99 HL119560 / NHLBI NIH HHS
Language: English
Date published: 2014
Academic Unit: Neurology; Molecular Physiology and Biophysics; Pulmonary, Critical Care, and Occupational Medicine; Neurosurgery; Internal Medicine
Record Identifier: 9984018033202771

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