Journal article
Expression and regulation of novel human beta-defensins in gingival keratinocytes
Oral microbiology and immunology, Vol.19(2), pp.111-117
04/2004
DOI: 10.1111/j.0902-0055.2002.00127.x
PMID: 14871351
Abstract
This study evaluated the expression and regulation of beta-defensins DEFB-104 and the recently identified DEFB-105-14 in gingival keratinocytes. Keratinocytes from healthy subjects were exposed to cytokines, Escherichia coli lipopolysaccharide or Candida species. Total RNA was extracted and defensin expression analyzed by reverse transcription-polymerase chain reaction. Three patterns of expression were seen: no expression, constitutive expression and inducible expression. Constitutive mRNA expression was evident for DEFB-104, 107, 109, 111, and 112. DEFB-108 and 114 were induced by interleukin (IL)-1beta and Candida species. For DEFB-108 expression, synergism was observed when IL-1beta was combined with tumor necrosis factor-alpha or interferon-gamma. Downregulation of DEFB-109 occurred following treatment with Candida albicans. These findings suggest a role for multiple beta-defensins in response to oral infection. Further investigation is needed to better understand their function, both in terms of antimicrobial activities and contributions to innate and acquired immunity.
Details
- Title: Subtitle
- Expression and regulation of novel human beta-defensins in gingival keratinocytes
- Creators
- P Premratanachai - Dows Institute for Dental Research, College of Dentistry, University of Iowa, Iowa City, IA 52242, USAS JolyG K JohnsonP B McCray JrH P JiaJ M Guthmiller
- Resource Type
- Journal article
- Publication Details
- Oral microbiology and immunology, Vol.19(2), pp.111-117
- Publisher
- Denmark
- DOI
- 10.1111/j.0902-0055.2002.00127.x
- PMID
- 14871351
- ISSN
- 0902-0055
- eISSN
- 1399-302X
- Grant note
- R01 DE 13334 / NIDCR NIH HHS
- Language
- English
- Date published
- 04/2004
- Academic Unit
- Microbiology and Immunology; Pulmonary Medicine; Stead Family Department of Pediatrics; Periodontics; Internal Medicine
- Record Identifier
- 9984065706802771
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