Expression and regulation of the human GLUT4/muscle-fat facilitative glucose transporter gene in transgenic mice

Min-Ling Liu; Ann Louise Olson; W. Scott Moye‐Rowley; John B Buse; Graeme I Bell; Jeffrey E Pessin

doi:10.1016/S0021-9258(19)49746-9

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Expression and regulation of the human GLUT4/muscle-fat facilitative glucose transporter gene in transgenic mice

Journal article

Open access

Peer reviewed

Expression and regulation of the human GLUT4/muscle-fat facilitative glucose transporter gene in transgenic mice

Min-Ling Liu, Ann Louise Olson, W. Scott Moye‐Rowley, John B Buse, Graeme I Bell and Jeffrey E Pessin

The Journal of biological chemistry, Vol.267(17), pp.11673-11676

06/15/1992

DOI: 10.1016/S0021-9258(19)49746-9

PMID: 1601840

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Abstract

To study the molecular basis of tissue-specific expression of the GLUT4/muscle-fat facilitative glucose transporter gene, we generated lines of transgenic mice carrying 2.4 kilobases of the 5'-flanking region of the human GLUT4 gene fused to a chloramphenicol acetyltransferase (CAT) reporter gene (hGLUT4[2.4]-CAT). This reporter gene construct was specifically expressed in tissues that normally express GLUT4 mRNA, which include both brown and white adipose tissues as well as cardiac, skeletal, and smooth muscle. In contrast, CAT reporter activity was not detected in brain or liver, two tissues that do not express the GLUT4 gene. In addition, the relative levels of CAT mRNA driven by the human GLUT4 promoter in various tissues of these transgenic animals mirrored those of the endogenous mouse GLUT4 mRNA. Since previous studies have observed alterations in GLUT4 mRNA levels induced by fasting and refeeding (Sivitz, W. I., DeSautel, S. L., Kayano, T., Bell, G. I., and Pessin, J. E. (1989) Nature 340, 72-74), the regulated expression the hGLUT4[2.4]-CAT transgene was also assessed in these animals. Fasting was observed to decrease CAT activity in white adipose tissue which was super-induced upon refeeding. These alterations in CAT expression occurred in parallel to the changes in endogenous mouse GLUT4 mRNA levels. Although CAT expression in skeletal muscle and brown adipose tissue was unaffected, the endogenous mouse GLUT4 mRNA was also refractory to the effects of fasting/refeeding in these tissues. These data demonstrate that 2.4 kilobases of the 5'-flanking region of the human GLUT4 gene contain all the necessary sequence elements to confer tissue-specific expression and at least some of the sequence elements controlling the hormonal/metabolic regulation of this gene.

Polymerase Chain Reaction

Adipose Tissue - metabolism

Animals

Base Sequence

Blotting, Northern

Chloramphenicol O-Acetyltransferase - genetics

Female

Gene Expression Regulation

Glucose Transporter Type 4

Humans

Mice

Mice, Transgenic

Molecular Sequence Data

Monosaccharide Transport Proteins - genetics

Muscle Proteins

Muscles - metabolism

Promoter Regions, Genetic

RNA, Messenger - metabolism

Starvation

Details

Title: Subtitle: Expression and regulation of the human GLUT4/muscle-fat facilitative glucose transporter gene in transgenic mice
Creators: Min-Ling Liu - University of Iowa
Ann Louise Olson - University of Iowa
W. Scott Moye‐Rowley - University of Iowa
John B Buse - University of Iowa
Graeme I Bell - University of Iowa
Jeffrey E Pessin - University of Iowa
Resource Type: Journal article
Publication Details: The Journal of biological chemistry, Vol.267(17), pp.11673-11676
DOI: 10.1016/S0021-9258(19)49746-9
PMID: 1601840
NLM abbreviation: J Biol Chem
ISSN: 0021-9258
eISSN: 1083-351X
Grant note: DK01822 / NIDDK NIH HHS DK42452 / NIDDK NIH HHS DK25925 / NIDDK NIH HHS
Language: English
Date published: 06/15/1992
Academic Unit: Molecular Physiology and Biophysics; Computer Science; Internal Medicine
Record Identifier: 9984297595902771

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