Expression of B cell surface receptors. II. IL-4 can accelerate the developmental expression of the murine B cell IgE Fc receptor

Thomas J. Waldschmidt; Daniel H Conrad; Richard G Lynch

doi:10.4049/jimmunol.143.9.2820

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Expression of B cell surface receptors. II. IL-4 can accelerate the developmental expression of the murine B cell IgE Fc receptor

Journal article

Peer reviewed

Expression of B cell surface receptors. II. IL-4 can accelerate the developmental expression of the murine B cell IgE Fc receptor

Thomas J. Waldschmidt, Daniel H Conrad and Richard G Lynch

The Journal of immunology (1950), Vol.143(9), pp.2820-2827

11/01/1989

DOI: 10.4049/jimmunol.143.9.2820

PMID: 2530279

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Abstract

The ability of IL-4 to influence the developmental expression of the murine B cell IgE Fc receptor (Fc epsilon R) was examined. Spleen cells from neonatal mice of increasing age were incubated overnight with IL-4 and subsequently examined with multicolor flow cytometry. The results demonstrate that IL-4 can significantly increase the number of maturing B cells which express the Fc epsilon R. This effect was only seen however, on those neonatal B cells which already displayed surface IgD. Splenic B cells which were IgM+, IgD- failed to express the Fc epsilon R when treated with IL-4, even though they responded by increasing their level of class II Ag expression. Further experiments showed that the inability of IgD- immature B cells to express the Fc epsilon R could not be entirely explained by their assignment to the Ly-1 lineage. Taken together, these results indicate that IL-4 can accelerate the developmental expression of the B cell Fc epsilon R, but only on those B cells that are mature enough to express IgD.

Flow Cytometry

Cell Differentiation

Immunoglobulin D - analysis

Spleen - immunology

Immunoglobulin M - analysis

Histocompatibility Antigens Class II - analysis

Antigens, Ly - analysis

Interleukin-4 - pharmacology

Animals

B-Lymphocytes - immunology

Receptors, Fc - immunology

Receptors, IgE

Antigens, Differentiation, B-Lymphocyte - immunology

Mice

Mice, Inbred BALB C

Animals, Newborn - immunology

Receptors, Antigen, B-Cell - analysis

Details

Title: Subtitle: Expression of B cell surface receptors. II. IL-4 can accelerate the developmental expression of the murine B cell IgE Fc receptor
Creators: Thomas J. Waldschmidt - Department of Pathology, University of Iowa College of Medicine, Iowa City 52242
Daniel H Conrad
Richard G Lynch
Resource Type: Journal article
Publication Details: The Journal of immunology (1950), Vol.143(9), pp.2820-2827
Publisher: United States
DOI: 10.4049/jimmunol.143.9.2820
PMID: 2530279
ISSN: 0022-1767
eISSN: 1550-6606
Grant note: CA 48485 / NCI NIH HHS AI 22600 / NIAID NIH HHS AI 18697 / NIAID NIH HHS
Language: English
Date published: 11/01/1989
Academic Unit: Pathology
Record Identifier: 9984047878702771

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