Expression of CD44 in human neoplastic and normal hyaline cartilage

Patrick P Bosch; Jeff W Stevens; Kenneth J Noonan; Joseph A Buckwalter; Ronald J Midura

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Expression of CD44 in human neoplastic and normal hyaline cartilage

Journal article

Open access

Peer reviewed

Expression of CD44 in human neoplastic and normal hyaline cartilage

Patrick P Bosch, Jeff W Stevens, Kenneth J Noonan, Joseph A Buckwalter and Ronald J Midura

The Iowa orthopaedic journal, Vol.22, pp.47-54

2002

PMCID: PMC1888362

PMID: 12180611

Appears in Diamond Open Access

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https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1888362/View

Published (Version of record) Open Access

Abstract

Although low-grade cartilage neoplasms typically consist of hyaline-like cartilage, most of them also contain some fibrocartilaginous regions. CD44, a cell surface receptor for hyaluronan, has been identified in cartilage. A family of alternatively spliced mRNA containing the variant 6 (v6) exon sequence of CD44 has been linked to several types of neoplasms. We hypothesized that expressions of v6-containing CD44 species are associated with fibrocartilaginous regions of low-grade cartilage neoplasms. To test this hypothesis we performed reverse transcriptase-polymerase chain reaction (RT-PCR) and immunohistochemical analysis on eight samples: four from normal articular cartilage, one from a synovial chondromatosis, and three chondrosarcomas which were graded as I and I/II. The standard CD44s and a unique v6-containing CD44 species (CD44v6-10) were identified in all tissue samples by RT-PCR analysis. Immunohistochemically, using an antibody that cross-reacted with all CD44 species, CD44 was localized to the cell surface, lacuna wall and intracellular compartment of the chondrocytes in the middle and deep zone of normal cartilage, as well as with cells throughout the neoplastic masses. Utilizing an antibody specific for v6-containing CD44 species, the variant species was identified throughout cells of the middle and deep zone of normal cartilage, and localized selectively to intracellular positions. In neoplastic masses, v6-containing CD44 species were found associated only with cells in the hyaline-like cartilage, but not in the fibrocartilaginous regions. Thus a differential expression of the v6-containing CD44 species in the neoplastic masses containing both hyaline-like cartilage and fibrocartilaginous regions was observed when compared to its homogenous expression in normal hyaline cartilage. An involvement between the lack of the variant CD44 (v6-containing) and altered tissue phenotype (e.g., fibrocartilaginous) is suggested.

Immunohistochemistry

Cartilage, Articular - metabolism

Glycoproteins - metabolism

Hyaluronan Receptors - metabolism

Osteochondroma - metabolism

Reverse Transcriptase Polymerase Chain Reaction

Cell Adhesion

Bone Neoplasms - metabolism

Details

Title: Subtitle: Expression of CD44 in human neoplastic and normal hyaline cartilage
Creators: Patrick P Bosch - Department of Orthopaedic Surgery, College of Medicine, The University of Iowa, Iowa City 52242-1181, USA
Jeff W Stevens
Kenneth J Noonan
Joseph A Buckwalter
Ronald J Midura
Resource Type: Journal article
Publication Details: The Iowa orthopaedic journal, Vol.22, pp.47-54
PMID: 12180611
PMCID: PMC1888362
NLM abbreviation: Iowa Orthop J
ISSN: 1541-5457
eISSN: 1555-1377
Publisher: Dept. of Orthopaedics, The University of Iowa; United States
Grant note: DK25295 / NIDDK NIH HHS AR-0705 / NIAMS NIH HHS
Language: English
Date published: 2002
Academic Unit: Stead Family Department of Pediatrics; Orthopedics and Rehabilitation; Injury Prevention Research Center
Record Identifier: 9984040593102771

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