Journal article
Expression of GB virus C NS5A protein from genotypes 1, 2, 3 and 5 and a 30 aa NS5A fragment inhibit human immunodeficiency virus type 1 replication in a CD4+ T-lymphocyte cell line
Journal of general virology, Vol.88(12), pp.3341-3346
2007
DOI: 10.1099/vir.0.83198-0
PMID: 18024904
Abstract
GB virus type C (GBV-C) is a common human flavivirus that has been associated with prolonged survival in HIV-positive individuals in several, though not all, epidemiological studies. There are five distinct GBV-C genotypes that are geographically localized, and it has been speculated that GBV-C genotypic differences may explain variable outcomes observed in different clinical studies. Expression of an 85 aa fragment of the GBV-C NS5A phosphoprotein (genotype 2) in a CD4+ T cell line (Jurkat) resulted in inhibition of HIV replication, mediated in part by decreased surface expression of the HIV coreceptor CXCR4 and upregulation of SDF-1. We expressed the NS5A protein from genotypes 1, 2, 3 and 5 in Jurkat cells, and demonstrated that all genotypes inhibited HIV replication. Further deletion mapping demonstrated that expression of a 30 aa fragment resulted in decreased CXCR4 surface expression, upregulation of SDF-1 and inhibition of HIV replication.
Details
- Title: Subtitle
- Expression of GB virus C NS5A protein from genotypes 1, 2, 3 and 5 and a 30 aa NS5A fragment inhibit human immunodeficiency virus type 1 replication in a CD4+ T-lymphocyte cell line
- Creators
- QING CHANG - Iowa City VA Medical Center and the University of Iowa, Iowa City, IA 52246, United StatesJames H MCLINDEN - Iowa City VA Medical Center and the University of Iowa, Iowa City, IA 52246, United StatesJack T STAPLETON - Iowa City VA Medical Center and the University of Iowa, Iowa City, IA 52246, United StatesM ASLAM SATHAR - Nelson R. Mandela School of Medicine, University of Kwazulu-Natal, South AfricaJINHUA XIANG - Iowa City VA Medical Center and the University of Iowa, Iowa City, IA 52246, United States
- Resource Type
- Journal article
- Publication Details
- Journal of general virology, Vol.88(12), pp.3341-3346
- Publisher
- Society for General Microbiology
- DOI
- 10.1099/vir.0.83198-0
- PMID
- 18024904
- ISSN
- 0022-1317
- eISSN
- 1465-2099
- Language
- English
- Date published
- 2007
- Academic Unit
- Microbiology and Immunology; Infectious Diseases; Internal Medicine
- Record Identifier
- 9984094332302771
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