Expression of IgG Fc receptor antigens in placenta and on endothelial cells in humans : an immunohistochemical study

D. D Sedmak; D. H Davis; UPINDER Singh; J. G. J VAN DE WINKEL; C. L Anderson

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Expression of IgG Fc receptor antigens in placenta and on endothelial cells in humans : an immunohistochemical study

Journal article

Peer reviewed

Expression of IgG Fc receptor antigens in placenta and on endothelial cells in humans : an immunohistochemical study

D. D Sedmak, D. H Davis, UPINDER Singh, J. G. J VAN DE WINKEL and C. L Anderson

The American journal of pathology, Vol.138(1), pp.175-181

1991

PMID: 1987763

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Abstract

Expression of leukocyte IgG Fc receptor (Fc gamma R) antigens by placenta, endothelial cells (EC) of normal tissues, and ECs of kidney and skin from subjects with immune complex diseases was studied immunohistochemically using anti-Fc gamma R monoclonal antibodies (MAb). Monoclonal antibodies against all three leukocyte Fc gamma R classes stained placental villous macrophages. Placental villous trophoblasts were stained intensely by anti-Fc gamma RIII MAb 3G8, while both anti-Fc gamma RI (MAb 32) and anti-Fc gamma RII MAbs IV3, KU79, CIKM5, 2E1, KB61, and 41H16) antibodies did not react with these cells. Anti-Fc gamma RII MAbs IV3, KU79, CIKM5, 2E1, KB61, and 41H16 immunostained placental villous capillary EC, in contrast to anti-Fc gamma RI MAb 32 and anti-Fc gamma RIII MAb 3G8, CLB-Granl, and B73.1, which did not bind. Anti-Fc gamma RI MAb 32, anti-Fc gamma RII MAb IV3 and CIKM5, and anti-Fc gamma RIII MAb 3G8 did not react with the ECs of tonsil, liver, kidney, spleen, intestine, lung, or uterus. Similarly no EC staining was seen with these four MAbs in 14 skin and 14 kidney biopsies from subjects with immune-complex diseases. Fc gamma R antigens are expressed constitutively only by placental villous ECs and are not induced on nonplacental ECs by immune-complex-mediated diseases.

Immunobiology

Biological and medical sciences

Fundamental and applied biological sciences. Psychology

Fundamental immunology

Immunological reactions in vitro

Lymphoblastic transformation (stimulation by antigen, mitogen, mixed lymphocyte reactions)

Details

Title: Subtitle: Expression of IgG Fc receptor antigens in placenta and on endothelial cells in humans : an immunohistochemical study
Creators: D. D Sedmak - The Ohio State University
D. H Davis - The Ohio State University
UPINDER Singh - The Ohio State University
J. G. J VAN DE WINKEL - The Ohio State University
C. L Anderson - The Ohio State University
Resource Type: Journal article
Publication Details: The American journal of pathology, Vol.138(1), pp.175-181
PMID: 1987763
NLM abbreviation: Am J Pathol
ISSN: 0002-9440
eISSN: 1525-2191
Publisher: American Society for Investigative Pathology
Number of pages: 7
Language: English
Date published: 1991
Academic Unit: Internal Medicine
Record Identifier: 9985244564102771

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