Journal article
Expression of Thrombomodulin and Consequences of Thrombomodulin Deficiency during Healing of Cutaneous Wounds
The American journal of pathology, Vol.155(5), pp.1569-1575
1999
DOI: 10.1016/S0002-9440(10)65473-9
PMCID: PMC1866991
PMID: 10550314
Abstract
Thrombomodulin is a cell surface anticoagulant that is expressed by endothelial cells and epidermal keratinocytes. Using immunohistochemistry, we examined thrombomodulin expression during healing of partial-thickness wounds in human skin and full-thickness wounds in mouse skin. We also examined thrombomodulin expression and wound healing in heterozygous thrombomodulin-deficient mice, compound heterozygous mice that have <1% of normal thrombomodulin anticoagulant activity, and chimeric mice derived from homozygous thrombomodulin-deficient embryonic stem cells. In both human and murine wounds, thrombomodulin was absent in keratinocytes at the leading edge of the neoepidermis, but it was expressed strongly by stratifying keratinocytes within the neoepidermis. No differences in rate or extent of reepithelialization were observed between wild-type and thrombomodulin-deficient mice. In chimeric mice, both thrombomodulin-positive and thrombomodulin-negative keratinocytes were detected within the neoepidermis. Compared with wild-type mice, heterozygous and compound heterozygous thrombomodulin-deficient mice exhibited foci of increased collagen deposition in the wound matrix. These findings demonstrate that expression of thrombomodulin in keratinocytes is regulated during cutaneous wound healing. Severe deficiency of thrombomodulin anticoagulant activity does not appear to alter reepithelialization but may influence collagen production by fibroblasts in the wound matrix.
Details
- Title: Subtitle
- Expression of Thrombomodulin and Consequences of Thrombomodulin Deficiency during Healing of Cutaneous Wounds
- Creators
- Jeffrey J Peterson - Department of Pathology, University of Iowa College of Medicine, Iowa City, IowaHelen B Rayburn - Department of Biology, Massachusetts Institute of Technology, Cambridge, MassachusettsDonna J Lager - Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, MinnesotaThomas J Raife - Blood Center of Southeastern Wisconsin, Milwaukee, WisconsinG. Patrick Kealey - Department of Surgery, University of Iowa College of Medicine, Iowa City, IowaRobert D Rosenberg - Department of Pathology, University of Iowa College of Medicine, Iowa City, IowaSteven R Lentz - Department of Internal Medicine, Veterans Affairs Medical Center, Iowa City, Iowa
- Resource Type
- Journal article
- Publication Details
- The American journal of pathology, Vol.155(5), pp.1569-1575
- Publisher
- Elsevier Inc
- DOI
- 10.1016/S0002-9440(10)65473-9
- PMID
- 10550314
- PMCID
- PMC1866991
- ISSN
- 0002-9440
- eISSN
- 1525-2191
- Grant note
- HL-07344 and DK-25295 / National Institutes of Health
- Language
- English
- Date published
- 1999
- Academic Unit
- Hematology, Oncology, and Blood & Marrow Transplantation; Surgery; Internal Medicine
- Record Identifier
- 9984094319302771
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