Journal article
Expression of ZER6 in ERα-Positive Breast Cancer
The Journal of surgical research, Vol.126(1), pp.86-91
2005
DOI: 10.1016/j.jss.2005.02.006
PMID: 15916980
Abstract
ZER6 is a C2H2 zinc finger transcription factor with two isoforms (p52-ZER6 and p71-ZER6), which are differentially repressed by a ligand-dependent interaction with estrogen receptor-alpha (ERα). We sought to determine if ZER6 proteins are expressed in ERα-positive breast cancer cells and if ZER6 is expressed in association with ERα in breast cancers.
The expression of ZER6 protein was examined by Western blot and the pattern of ZER6 expression was examined in a panel of ERα-positive and ERα-negative breast cancers using RT-PCR.
COS-1 cells transfected with expression vectors for p52-ZER6 express a major protein of 52 kDa and a minor protein of 75 kDa, whereas cells transfected with the p71-ZER6 expression vector express a major protein of 77 kDa and a minor protein of 100 kDa. Breast carcinoma cells express ZER6-specific proteins of similar size, and expression of the p52-ZER6 isoform was only detected in the ERα-positive cell lines. In primary breast cancer tissue, 8/16 (50%) of the ERα-positive tumors had high ZER6 expression, whereas only 1/12 (8%) of the ERα-negative tumors had a high ZER6 level of expression. The relative abundance of ZER6 mRNA in the ERα-positive group was statistically greater than the ERα-negative group (188
versus 106,
P < 0.05).
We have confirmed that breast carcinoma cells express ZER6 proteins and identified an association between the level of ZER6 expression and ERα expression in primary breast cancers. These data support a role for the ZER6 transcription factors in regulating the expression of genes in hormone-responsive breast cancer.
Details
- Title: Subtitle
- Expression of ZER6 in ERα-Positive Breast Cancer
- Creators
- Paul R StabachManimekalai M ThiyagarajanRonald J Weigel
- Resource Type
- Journal article
- Publication Details
- The Journal of surgical research, Vol.126(1), pp.86-91
- Publisher
- Elsevier Inc
- DOI
- 10.1016/j.jss.2005.02.006
- PMID
- 15916980
- ISSN
- 0022-4804
- eISSN
- 1095-8673
- Language
- English
- Date published
- 2005
- Academic Unit
- Molecular Physiology and Biophysics; Anatomy and Cell Biology; Surgery; Biochemistry and Molecular Biology
- Record Identifier
- 9984024543102771
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