Expression of a D1 dopamine receptor dDA1/DmDOP1 in the central nervous system of Drosophila melanogaster

Young-Cho Kim; Hyun-Gwan Lee; Chang-Soo Seong; Kyung-An Han

doi:10.1016/S1567-133X(02)00098-4

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Expression of a D1 dopamine receptor dDA1/DmDOP1 in the central nervous system of Drosophila melanogaster

Journal article

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Expression of a D1 dopamine receptor dDA1/DmDOP1 in the central nervous system of Drosophila melanogaster

Young-Cho Kim, Hyun-Gwan Lee, Chang-Soo Seong and Kyung-An Han

Gene expression patterns : GEP, Vol.3(2), pp.237-245

05/2003

DOI: 10.1016/S1567-133X(02)00098-4

PMID: 12711555

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Abstract

The diverse physiological effects of dopamine are mediated by multiple receptor systems. The dDA1 represents one of the Drosophila dopamine receptors that activate the cAMP cascade. To gain insight into the role of dDA1, we generated a polyclonal antibody against the unique sequence in dDA1 and investigated dDA1 distribution in the central nervous system (CNS) of Drosophila melanogaster. In both larval and adult CNS pronounced dDA1 immunoreactivity was present in the neuropil of the mushroom bodies, a brain structure crucial for learning and memory in insects, and four unpaired neurons in each thoracic segment. In addition, the larval abdominal ganglion contained two dDA1 cells in each segment. This expression pattern appeared to be maintained in the condensed adult abdominal ganglion although the precise number and the intensity of staining were somewhat variable. The adult CNS also exhibited intense dDA1 immunoreactivity in the central complex, a structure controlling higher-order motor function, moderate expression in several neurosecretory cells, and weak staining in two unpaired neurons in the mesothoracic neuromere. The dDA1 expression in these areas was only detected in adult, but not in third instar larval CNS.

Brain - embryology

Drosophila melanogaster - embryology

Central Nervous System - metabolism

Membrane Proteins - genetics

Larva - metabolism

Gene Expression Profiling

Drosophila melanogaster - genetics

Drosophila Proteins - biosynthesis

Brain - metabolism

Drosophila melanogaster - metabolism

Membrane Proteins - biosynthesis

Animals

Fluorescent Antibody Technique

Drosophila Proteins - genetics

Receptors, Dopamine - genetics

Receptors, Dopamine - biosynthesis

Details

Title: Subtitle: Expression of a D1 dopamine receptor dDA1/DmDOP1 in the central nervous system of Drosophila melanogaster
Creators: Young-Cho Kim - Department of Biobehavioral Health and The Huck Institute for Life Sciences, 315 Health and Human Development East, Pennsylvania State University, University Park, PA 16802, USA
Hyun-Gwan Lee
Chang-Soo Seong
Kyung-An Han
Resource Type: Journal article
Publication Details: Gene expression patterns : GEP, Vol.3(2), pp.237-245
DOI: 10.1016/S1567-133X(02)00098-4
PMID: 12711555
NLM abbreviation: Gene Expr Patterns
ISSN: 1567-133X
eISSN: 1872-7298
Publisher: Netherlands
Grant note: R01 NS38346 / NINDS NIH HHS
Language: English
Date published: 05/2003
Academic Unit: Neurology; Iowa Neuroscience Institute
Record Identifier: 9984020781702771

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