Expression of a single βα chain protein of equine LH/CG in milk of transgenic rabbits and its biological activity

C. Galet; C. Menck Le Bourhis; M. Chopineau; G. Le Griec; A. Perrin; T. Magallon; J. Attal; C. Viglietta; L. M. Houdebine; F. Guillou

doi:10.1016/S0303-7207(00)00452-4

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Expression of a single βα chain protein of equine LH/CG in milk of transgenic rabbits and its biological activity

Journal article

Peer reviewed

Expression of a single βα chain protein of equine LH/CG in milk of transgenic rabbits and its biological activity

C. Galet, C. Menck Le Bourhis, M. Chopineau, G. Le Griec, A. Perrin, T. Magallon, J. Attal, C. Viglietta, L. M. Houdebine and F. Guillou

Molecular and cellular endocrinology, Vol.174(1-2), pp.31-40

03/2001

DOI: 10.1016/S0303-7207(00)00452-4

PMID: 11306169

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Abstract

Equine chorionic gonadotropin (eCG) is a heavily glycosylated glycoprotein composed of non-covalently linked α- and β-subunits. eCG possesses the particularity to bind to both LH and FSH receptors in species other than horses and to have a prolonged plasma half-life. All these properties make it of utmost interest for livestock fertilization program. Up to now, the only source of eCG is the serum of pregnant mare. Rabbit mammary gland is considered as a system able to produce recombinant glycoproteins in sufficient quantity for pharmaceutical use. Here we described the production of a recombinant single βα chain of eLH/CG in the milk of transgenic rabbit. The construction of a single-chain permits to by-pass the problem of association-dissociation of the subunits. This recombinant hormone is greatly expressed (21.7 mg/l) and presents similar in vitro LH and FSH bioactivities. However, βαeLH/CG shows an extremely rapid clearance (≈10 min), which could explain the absence of in vivo biological activity. So the rabbit mammary gland is not appropriate for the production of a recombinant active eLH/CG. Copyright © 2001 Elsevier Science Ireland Ltd.

Biological activities

eCG

Milk expression

Transgenic rabbits

Details

Title: Subtitle: Expression of a single βα chain protein of equine LH/CG in milk of transgenic rabbits and its biological activity
Creators: C. Galet - Centre National de la Recherche Scientifique
C. Menck Le Bourhis - Société Bioprotein Technologie, France
M. Chopineau - Physiologie de la Reproduction et des Comportements
G. Le Griec - Société Bioprotein Technologie, France
A. Perrin - Société Bioprotein Technologie, France
T. Magallon - Physiologie de la Reproduction et des Comportements
J. Attal - Virologie et Immunologie Moléculaires
C. Viglietta - Virologie et Immunologie Moléculaires
L. M. Houdebine - Virologie et Immunologie Moléculaires
F. Guillou - Université de Tours
Resource Type: Journal article
Publication Details: Molecular and cellular endocrinology, Vol.174(1-2), pp.31-40
DOI: 10.1016/S0303-7207(00)00452-4
PMID: 11306169
NLM abbreviation: Mol Cell Endocrinol
ISSN: 0303-7207
eISSN: 1872-8057
Publisher: ELSEVIER SCI IRELAND LTD; CLARE
Number of pages: 10
Grant note: Institut National de la Recherche Agronomique (http://data.elsevier.com/vocabulary/SciValFunders/501100006488) Region Centre
Language: English
Date published: 03/2001
Academic Unit: Injury Prevention Research Center; University of Iowa Health Care
Record Identifier: 9985138031402771

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