Journal article
Expression of beta-defensins in gingival health and in periodontal disease
Journal of oral pathology & medicine, Vol.33(5), pp.278-285
05/2004
DOI: 10.1111/j.0904-2512.2004.00143.x
PMID: 15078488
Abstract
Human beta-defensins (HBDs) are epithelial-derived antimicrobial peptides. The expression of three HBDs (HBD-1, HBD-2, and HBD-3) has been reported in oral mucosa, gingiva, and salivary glands. However, their role in protection against oral infections is not well understood. This study examined the expression of HBD-1, HBD-2, and HBD-3 mRNAs in gingival health and periodontal disease.
Gingival tissue discarded from periodontal procedures was obtained from 20 periodontally healthy and 29 periodontally diseased sites. Total RNA was isolated, and expression of beta-defensins was analyzed by both 35-cycle and semiquantitative (25-cycle) reverse transcription-polymerase chain reaction (RT-PCR). The level of expression was assigned ordinal scores of no expression, low expression, or high expression. mRNA expression was compared between healthy and diseased groups using exact tests of homogeneity and Cochran-Mantel-Haenszel tests; associations among the variables were assessed using exact tests and Kendall's tau-b statistic.
All 49 samples demonstrated basal mRNA expression of HBD-1, HBD-2, and HBD-3. Significantly higher levels of HBD-3 (P = 0.012) expression were found in the healthy tissues as compared to the diseased ones. There was also a suggestion of higher expression of HBD-2 in the healthy tissues (P = 0.12). Levels of HBD-1, HBD-2, and HBD-3 mRNA expression were correlated with one another (P < 0.001).
High levels of HBD-3 mRNA expression in healthy tissues suggest a potentially important protective role for defensins in the host immune response to infection by periodontal pathogens.
Details
- Title: Subtitle
- Expression of beta-defensins in gingival health and in periodontal disease
- Creators
- John Bissell - Dows Institute for Dental Research, Department of Periodontics, College of Dentistry, University of Iowa, IA 52242, USASophie JolyGeorgia K JohnsonConnie C OrganDeborah DawsonPaul B McCray JrJanet M Guthmiller
- Resource Type
- Journal article
- Publication Details
- Journal of oral pathology & medicine, Vol.33(5), pp.278-285
- Publisher
- Denmark
- DOI
- 10.1111/j.0904-2512.2004.00143.x
- PMID
- 15078488
- ISSN
- 0904-2512
- eISSN
- 1600-0714
- Grant note
- 1R01 DE13334 / NIDCR NIH HHS
- Language
- English
- Date published
- 05/2004
- Academic Unit
- Microbiology and Immunology; Pulmonary Medicine; Stead Family Department of Pediatrics; Biostatistics; Pediatric Dentistry; Periodontics; Internal Medicine
- Record Identifier
- 9984065980102771
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