Journal article
Expression of both B7-1 and CD28 contributes to the IL-2 responsiveness of CTLL-2 cells
Immunology, Vol.87(2), pp.271-274
02/1996
DOI: 10.1046/j.1365-2567.1996.461532.x
PMCID: PMC1384284
PMID: 8698390
Abstract
The CTLL-2 bioassay is used frequently to determine interleukin-2 (IL-2) concentrations in experimental samples, including samples that contain reagents which affect the CD28-B7 interaction. We therefore evaluated whether the CD28-B7 pathway plays a role in the growth of CTLL-2 cells. Flow cytometry demonstrated that CTLL-2 cells express both CD28 and B7-1. CTLA4-immunoglobulin (CTLA4-Ig) inhibited the growth of CTLL-2 cells over a range of IL-2 concentrations, suggesting that the CD28-B7 interaction plays an important role in the growth of CTLL-2 cells. Anti-B7-1 antibody also inhibited CTLL-2 proliferation at all concentrations of IL-2. These results indicate that the CTLL-2 bioassay may not be a reliable means of determining IL-2 levels in experimental samples containing reagents that affect the CD28-B7 interaction. They also suggest that co-expression of CD28 and B7 may contribute to the growth of malignant T cells.
Details
- Title: Subtitle
- Expression of both B7-1 and CD28 contributes to the IL-2 responsiveness of CTLL-2 cells
- Creators
- R Belani - University of Iowa, Iowa City, 52242, USAG J Weiner
- Resource Type
- Journal article
- Publication Details
- Immunology, Vol.87(2), pp.271-274
- DOI
- 10.1046/j.1365-2567.1996.461532.x
- PMID
- 8698390
- PMCID
- PMC1384284
- NLM abbreviation
- Immunology
- ISSN
- 0019-2805
- eISSN
- 1365-2567
- Grant note
- CA55178 / NCI NIH HHS
- Language
- English
- Date published
- 02/1996
- Academic Unit
- Hematology, Oncology, and Blood & Marrow Transplantation; Pharmaceutical Sciences and Experimental Therapeutics; Internal Medicine
- Record Identifier
- 9984094754002771
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