Journal article
Expression of cystic fibrosis transmembrane conductance regulator in a model epithelium
American journal of physiology. Lung cellular and molecular physiology, Vol.266(4), pp.L405-L413
04/01/1994
DOI: 10.1152/ajplung.1994.266.4.L405
PMID: 7513963
Abstract
Cystic fibrosis transmembrane conductance regulator (CFTR) is a Cl- channel regulated by adenosine 3',5'-cyclic monophosphate (cAMP)-dependent phosphorylation and by intracellular nucleotides. The function of CFTR, like other recombinant ion channels, has generally been studied in single cells using voltage-clamp techniques. However, because CFTR is normally located in the apical membrane of epithelia we wanted to develop a system to study the function of recombinant CFTR expressed in an epithelium. We chose Fischer rat thyroid (FRT) epithelia for two reasons. First, when grown on permeable filter supports, FRT cells form polarized epithelia with a high transepithelial resistance. Second, they have no endogenous cAMP-regulated Cl- channels in their apical membrane. We expressed CFTR in FRT epithelia either transiently, using recombinant vaccinia virus, or stably, using a retrovirus. To measure apical membrane Cl- currents, we permeabilized the basolateral membrane to monovalent ions with nystatin and imposed a large transepithelial Cl- concentration gradient. cAMP agonists stimulated apical membrane Cl- currents in FRT epithelia infected with wild-type CFTR (vTF-CFTR) but not in FRT epithelia infected with either control virus (vTF7-3) or CFTR containing the delta F508 mutation (vTF-delta F508). These Cl- currents had properties similar to those of cAMP-activated Cl- currents in cells expressing endogenous or recombinant CFTR.
Details
- Title: Subtitle
- Expression of cystic fibrosis transmembrane conductance regulator in a model epithelium
- Creators
- David N Sheppard - Howard Hughes Medical Institute, Department of Internal Medicine,University of Iowa College of Medicine, Iowa City 52242Mark R Carson - Howard Hughes Medical Institute, Department of Internal Medicine,University of Iowa College of Medicine, Iowa City 52242Lynda S Ostedgaard - Howard Hughes Medical Institute, Department of Internal Medicine,University of Iowa College of Medicine, Iowa City 52242Gerene M Denning - Howard Hughes Medical Institute, Department of Internal Medicine,University of Iowa College of Medicine, Iowa City 52242Michael J Welsh - Howard Hughes Medical Institute, Department of Internal Medicine,University of Iowa College of Medicine, Iowa City 52242
- Resource Type
- Journal article
- Publication Details
- American journal of physiology. Lung cellular and molecular physiology, Vol.266(4), pp.L405-L413
- DOI
- 10.1152/ajplung.1994.266.4.L405
- PMID
- 7513963
- NLM abbreviation
- Am J Physiol Lung Cell Mol Physiol
- ISSN
- 1040-0605
- eISSN
- 1522-1504
- Publisher
- American Physiological Society
- Language
- English
- Date published
- 04/01/1994
- Academic Unit
- Neurology; Molecular Physiology and Biophysics; Pulmonary, Critical Care, and Occupational Medicine; Emergency Medicine; Fraternal Order of Eagles Diabetes Research Center; Neurosurgery; Internal Medicine
- Record Identifier
- 9984020655302771
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