Journal article
Expression of dystrophin-associated glycoproteins and utrophin in carriers of Duchenne muscular dystrophy
Neuromuscular disorders : NMD, Vol.4(5), pp.401-409
1994
DOI: 10.1016/0960-8966(94)90079-5
PMID: 7881285
Abstract
The expression of dystrophin, the dystrophin-associated proteins and utrophin has been studied immunocytochemically in three young, manifesting carriers of Duchenne muscular dystrophy, aged 3, 5 and 12 yrs, one adult manifesting carrier, aged 60 yrs, and one presumptive carrier with a raised serum creatine kinase, aged 24 yrs, the mother of the 5-yr-old manifesting carrier. The manifesting carriers had variable degrees of weakness; the presumptive carrier had no weakness. Morphological abnormalities were also variable and were most marked in the young manifesting carriers. The three young manifesting carriers and the presumptive carrier had a mosaic pattern of dystrophin-positive and dystrophin-negative fibres. All the dystrophin-associated proteins were reduced in the dystrophin-deficient fibres, giving a similar mosaic pattern to dystrophin. Expression of dystrophin and the dystrophin-associated proteins was normal in the adult manifesting carrier. Utrophin was detected on the sarcolemma of fibres both with and without dystrophin and the dystrophin-associated proteins. Thus, dystrophin and utrophin are co-expressed in several fibres in carriers. The results emphasize the close association between dystrophin and the glycoprotein complex and their role in the pathogenesis of muscle damage. In addition, the presence of utrophin in fibres with greatly reduced glycoproteins suggests that very little of the glycoprotein complex may be required to anchor the amount of utrophin expressed at the sarcolemma in these particular cases.
Details
- Title: Subtitle
- Expression of dystrophin-associated glycoproteins and utrophin in carriers of Duchenne muscular dystrophy
- Creators
- C.A Sewry - Neuromuscular Unit, Department of Paediatrics and Neonatal Medicine, Royal Postgraduate Medical School, Hammersmith Hospital, Du Cane Road, London W12 0NN, U.KK Matsumura - Howard Hughes Medical Institute and Department of Physiology and Biophysics, University of Iowa, 400 EMRB, Iowa City, IA 52242, U.S.AK.P Campbell - Howard Hughes Medical Institute and Department of Physiology and Biophysics, University of Iowa, 400 EMRB, Iowa City, IA 52242, U.S.AV Dubowitz - Neuromuscular Unit, Department of Paediatrics and Neonatal Medicine, Royal Postgraduate Medical School, Hammersmith Hospital, Du Cane Road, London W12 0NN, U.K
- Resource Type
- Journal article
- Publication Details
- Neuromuscular disorders : NMD, Vol.4(5), pp.401-409
- Publisher
- Elsevier B.V
- DOI
- 10.1016/0960-8966(94)90079-5
- PMID
- 7881285
- ISSN
- 0960-8966
- eISSN
- 1873-2364
- Language
- English
- Date published
- 1994
- Academic Unit
- Neurology; Molecular Physiology and Biophysics; Iowa Neuroscience Institute
- Record Identifier
- 9984068382902771
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