Journal article
Expression of foreign genes in adult rats' central nervous system by use of defective herpes simplex virus type 1 vectors
Zhonghua shi yan he lin chuang bing du xue za ji, Vol.11(4), pp.306-310
12/01/1997
PMID: 15617234
Abstract
We developed a defective herpes simplex virus (HSV) vector system that permitted the introduction of virtually any gene into mammalian central nervous system neurons. The HSV-1 amplicon plasmid, pHSL, contained a beta-galactosidase transcription cassette directed by HSV-1 IE68 promoter and two elements from HSV-1 genome: the origin of HSV-1 replication and its packaging site sequence. Being transfected into immortalized cells such as Vero cells in the present of HSV-1 to provide the helper virus function, (in our tests, a temperature-sensitive mutank tsK strain with permissive temperature at 31 degrees C was used), the prototype vector pHSL could be packaged into HSV-1 particles as a head-to-tail concatemer with the size of about the genome of HSV-1. A mixed HSV-1 stocks obtained were called dvHSL. Infection of cultured embryonic spinal cord motor neurons and forebrain cortex neurons of rats with dvHSL showed that beta-galactosidase was expressed up to twelve days. Via corneal inoculation, the trigeminal gang
Details
- Title: Subtitle
- Expression of foreign genes in adult rats' central nervous system by use of defective herpes simplex virus type 1 vectors
- Creators
- T YangX WangZ YanY Hou
- Resource Type
- Journal article
- Publication Details
- Zhonghua shi yan he lin chuang bing du xue za ji, Vol.11(4), pp.306-310
- PMID
- 15617234
- ISSN
- 1003-9279
- Language
- Chinese
- Date published
- 12/01/1997
- Academic Unit
- Anatomy and Cell Biology
- Record Identifier
- 9984284357302771
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