Expression of glutathione S-transferases in poplar trees (Populus trichocarpa) exposed to 2,4,6-trinitrotoluene (TNT)

Laura B Brentner; Sachiyo T Mukherji; Kate M Merchie; Jong Moon Yoon; Jerald L Schnoor; Benoit Van Aken

doi:10.1016/j.chemosphere.2008.07.059

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Expression of glutathione S-transferases in poplar trees (Populus trichocarpa) exposed to 2,4,6-trinitrotoluene (TNT)

Journal article

Peer reviewed

Expression of glutathione S-transferases in poplar trees (Populus trichocarpa) exposed to 2,4,6-trinitrotoluene (TNT)

Laura B Brentner, Sachiyo T Mukherji, Kate M Merchie, Jong Moon Yoon, Jerald L Schnoor and Benoit Van Aken

Chemosphere (Oxford), Vol.73(5), pp.657-662

2008

DOI: 10.1016/j.chemosphere.2008.07.059

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Abstract

Twelve Populus genes were identified from Arabidopsis thaliana sequences previously shown to be induced by exposure to 2,4,6-trinitrotoluene (TNT). Using the resources of the Poplar Genome Project and National Center for Biotechnology Information databases, Populus conserved domains were identified and used to design gene specific primers. RNA extracted from root tissues of TNT-exposed hydroponic poplar plants was used to quantify the expression of genes by reverse-transcriptase real-time polymerase chain reaction. Cyclophilin and 18S ribosomal DNA genes were used as internal standards. Exposure to TNT resulted in a significant increase of gene expression of two glutathione S-transferases (GST), peaking at levels of 25.0 ± 13.1 and 10 ± 0.7 fold the expression level of non-exposed plants after 24 h for each of the GST genes, respectively. This paper demonstrates the use of functional genomics information from the model plant species, Arabidopsis, to identify genes which may be important in detoxification of TNT in the model phytoremediation species, Populus trichocarpa.

Gene Expression

Genes

Genomics

abiotic stress

forest trees

gene expression regulation

genome

glutathione transferase

hydroponics

metabolic detoxification

molecular sequence data

nucleotide sequences

phytoremediation

Populus balsamifera subsp. trichocarpa

reverse transcriptase polymerase chain reaction

ribosomal DNA

roots

trinitrotoluene

Details

Title: Subtitle: Expression of glutathione S-transferases in poplar trees (Populus trichocarpa) exposed to 2,4,6-trinitrotoluene (TNT)
Creators: Laura B Brentner
Sachiyo T Mukherji
Kate M Merchie
Jong Moon Yoon - Iowa State University
Jerald L Schnoor - University of Iowa, Civil and Environmental Engineering
Benoit Van Aken - Temple University
Resource Type: Journal article
Publication Details: Chemosphere (Oxford), Vol.73(5), pp.657-662
DOI: 10.1016/j.chemosphere.2008.07.059
ISSN: 0045-6535
eISSN: 1879-1298
Language: English
Date published: 2008
Academic Unit: Civil and Environmental Engineering; Occupational and Environmental Health
Record Identifier: 9983557543002771

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