Expression of growth-associated protein-43 kD in Schwann cells is regulated by axon-Schwann cell interactions and cAMP

S S Scherer; Y T Xu; D Roling; L Wrabetz; M L Feltri; J Kamholz

doi:10.1002/jnr.490380510

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Expression of growth-associated protein-43 kD in Schwann cells is regulated by axon-Schwann cell interactions and cAMP

Journal article

Peer reviewed

Expression of growth-associated protein-43 kD in Schwann cells is regulated by axon-Schwann cell interactions and cAMP

S S Scherer, Y T Xu, D Roling, L Wrabetz, M L Feltri and J Kamholz

Journal of neuroscience research, Vol.38(5), pp.575-589

08/01/1994

DOI: 10.1002/jnr.490380510

PMID: 7815473

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Abstract

We have examined the regulation of growth-associated protein 43 kD (GAP-43) in rat Schwann cells. In unlesioned adult nerves, GAP-43-immunoreactivity was restricted to non-myelinating Schwann cells and unmyelinated axons. When adult nerves were transected to cause permanent axotomy, previously myelinating Schwann cells expressed progressively more GAP-43-immunoreactivity over 3 weeks, and GAP-43 mRNA levels increased over a similar time course. The peak level of GAP-43 mRNA occurred at least 2 weeks later than that of nerve growth factor receptor, another marker of denervated Schwann cells. In contrast, after nerve-crush, which allows axonal regeneration, many fewer Schwann cells had GAP-43-immunoreactivity, and the amount of GAP-43 mRNA was markedly lower than in transected nerves. Forskolin, a drug that activates adenylate cyclase and mimics many effects of axon-Schwann cell interactions, markedly reduced GAP-43-immunoreactivity and mRNA expression in cultured Schwann cells, whereas interleukin-1 had no effect. These data demonstrate that axon-Schwann cell interactions inhibit the expression of GAP-43 in Schwann cells and that this effect is mimicked by forskolin.

Sciatic Nerve - chemistry

Sciatic Nerve - physiology

Sciatic Nerve - injuries

Membrane Glycoproteins - biosynthesis

GAP-43 Protein

Axons - physiology

Schwann Cells - cytology

Cyclic AMP - physiology

Wallerian Degeneration

RNA, Messenger - biosynthesis

Nerve Tissue Proteins - biosynthesis

Schwann Cells - drug effects

Colforsin - pharmacology

Cells, Cultured

Nerve Crush

Cell Communication

Gene Expression Regulation - physiology

Rats

Schwann Cells - metabolism

Nerve Tissue Proteins - genetics

Rats, Sprague-Dawley

Membrane Glycoproteins - genetics

Blotting, Northern

Animals

Fibroblasts - drug effects

Details

Title: Subtitle: Expression of growth-associated protein-43 kD in Schwann cells is regulated by axon-Schwann cell interactions and cAMP
Creators: S S Scherer - Department of Neurology, University of Pennsylvania, Philadelphia 19104-6146
Y T Xu
D Roling
L Wrabetz
M L Feltri
J Kamholz
Resource Type: Journal article
Publication Details: Journal of neuroscience research, Vol.38(5), pp.575-589
Publisher: United States
DOI: 10.1002/jnr.490380510
PMID: 7815473
ISSN: 0360-4012
eISSN: 1097-4547
Grant note: NS-08075 / NINDS NIH HHS NS-01565 / NINDS NIH HHS
Language: English
Date published: 08/01/1994
Academic Unit: Neurology; Psychiatry
Record Identifier: 9984020881202771

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