Journal article
Expression of human paraoxonase 1 decreases superoxide levels and alters bacterial colonization in the gut of Drosophila melanogaster
PloS one, Vol.7(8), pp.e43777-e43777
2012
DOI: 10.1371/journal.pone.0043777
PMCID: PMC3431398
PMID: 22952763
Abstract
Paraoxonases (PON) are a family of proteins (PON1, 2 and 3) with multiple enzymatic activities. PON1 interferes with homoserine lactone-mediated quorum sensing in bacteria and with reactive oxygen species (ROS) in humans and mice. PON1 gene mutations have been linked to multiple traits, including aging, and diseases of the cardiovascular, nervous and gastrointestinal system. The overlapping enzymatic activities in the PON family members and high linkage disequilibrium rates within their polymorphisms confound animal and human studies of PON1 function. In contrast, arthropods such as Drosophila melanogaster have no PON homologs, resulting in an ideal model to study interactions between PON genotype and host phenotypes. We hypothesized that expression of PON1 in D. melanogaster would alter ROS. We found that PON1 alters expression of multiple oxidative stress genes and decreases superoxide anion levels in normal and germ-free D. melanogaster. We also found differences in the composition of the gut microbiota, with a remarkable increase in levels of Lactobacillus plantarum and associated changes in expression of antimicrobial and cuticle-related genes. PON1 expression directly decreased superoxide anion levels and altered bacterial colonization of the gut and its gene expression profile, highlighting the complex nature of the interaction between host genotype and gut microbiota. We speculate that the interaction between some genotypes and human diseases may be mediated by the presence of certain gut bacteria that can induce specific immune responses in the gut and other host tissues.
Details
- Title: Subtitle
- Expression of human paraoxonase 1 decreases superoxide levels and alters bacterial colonization in the gut of Drosophila melanogaster
- Creators
- Alejandro A Pezzulo - Department of Internal Medicine, Carver College of Medicine, University of Iowa, Iowa City, Iowa, United States of AmericaEmma E HornickMichael V RectorMiriam EstinAnna C ReisetterPeter J TaftStephen C ButcherA Brent CarterJ Robert ManakDavid A StoltzJoseph Zabner
- Resource Type
- Journal article
- Publication Details
- PloS one, Vol.7(8), pp.e43777-e43777
- DOI
- 10.1371/journal.pone.0043777
- PMID
- 22952763
- PMCID
- PMC3431398
- NLM abbreviation
- PLoS One
- ISSN
- 1932-6203
- eISSN
- 1932-6203
- Publisher
- Public Library of Science; United States
- Grant note
- HL091842-02 / NHLBI NIH HHS ES015981 / NIEHS NIH HHS P01 HL091842 / NHLBI NIH HHS R01 ES015981 / NIEHS NIH HHS I01 BX001135 / BLRD VA ES014871 / NIEHS NIH HHS R01 ES014871 / NIEHS NIH HHS
- Language
- English
- Date published
- 2012
- Academic Unit
- Roy J. Carver Department of Biomedical Engineering; Molecular Physiology and Biophysics; Pulmonary, Critical Care, and Occupational Medicine; Microbiology and Immunology; Stead Family Department of Pediatrics; Iowa Neuroscience Institute; Biology; Radiation Oncology; Craniofacial Anomalies Research Center; Internal Medicine
- Record Identifier
- 9984025421902771
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