Expression of inducible nitric oxide synthase in the lower esophageal sphincter of the endotoxemic opossum

Hyojin Park; Eugene Clark; Joseph J Cullen; John G Koland; Myong Soo Kim; Jeffrey L Conklin

doi:10.1007/s005350200169

Back

Expression of inducible nitric oxide synthase in the lower esophageal sphincter of the endotoxemic opossum

Journal article

Peer reviewed

Expression of inducible nitric oxide synthase in the lower esophageal sphincter of the endotoxemic opossum

Hyojin Park, Eugene Clark, Joseph J Cullen, John G Koland, Myong Soo Kim and Jeffrey L Conklin

Journal of gastroenterology, Vol.37(12), pp.1000-1004

2002

DOI: 10.1007/s005350200169

PMID: 12522530

Files and links (1)

url

https://ir.ymlib.yonsei.ac.kr/handle/22282913/143930View

Open Access

Abstract

Endotoxin modulates esophageal motor function by increasing nitric oxide (NO) production. The aims of this study were to examine inducible nitric oxide synthase (iNOS) induction in the lower esophageal sphincter (LES) of endotoxemic opossums and to investigate the effects of aminoguanidine (AG), a selective inhibitor of iNOS, on plasma nitrite/nitrate levels and on iNOS protein and mRNA expression after exposure to lipopolysaccharide (LPS). Before and 12 h after the intravenous administration of LPS and/or AG, plasma nitrite/nitrate levels were determined. The iNOS protein and mRNA expression were investigated in the tissues taken from the LES by Western blot and reverse-transcriptase polymerase chain reaction (RT-PCR). Plasma nitrite/nitrate levels were significantly increased by LPS. The increase in plasma nitrite/nitrate produced by LPS was significantly decreased by AG. Western blot and RT-PCR demonstrated that iNOS expression was markedly increased by LPS, and attenuated slightly by AG. These studies support the hypothesis that endotoxin increases NO production by the induction of iNOS protein and mRNA.

Esophagogastric Junction - drug effects

Endotoxemia - enzymology

Esophagogastric Junction - physiopathology

Opossums

Molecular Sequence Data

RNA, Messenger - analysis

Guanidines - pharmacology

Male

Nitric Oxide Synthase - genetics

Nitric Oxide - analysis

Nitric Oxide Synthase Type II

Lipopolysaccharides

Base Sequence

Endotoxemia - physiopathology

Sensitivity and Specificity

Statistics, Nonparametric

Female

Disease Models, Animal

Gene Expression Regulation

Probability

Random Allocation

Reverse Transcriptase Polymerase Chain Reaction

Blotting, Western

Animals

Nitric Oxide Synthase - metabolism

Nitric Oxide - metabolism

Details

Title: Subtitle: Expression of inducible nitric oxide synthase in the lower esophageal sphincter of the endotoxemic opossum
Creators: Hyojin Park - Department of Internal Medicine, University of Iowa College of Medicine and VA Medical Center, Iowa City, USA
Eugene Clark
Joseph J Cullen
John G Koland
Myong Soo Kim
Jeffrey L Conklin
Resource Type: Journal article
Publication Details: Journal of gastroenterology, Vol.37(12), pp.1000-1004
DOI: 10.1007/s005350200169
PMID: 12522530
NLM abbreviation: J Gastroenterol
ISSN: 0944-1174
eISSN: 1435-5922
Publisher: Japan
Language: English
Date published: 2002
Academic Unit: Surgery; Radiation Oncology; Neuroscience and Pharmacology
Record Identifier: 9984051531202771

Metrics

24 Record Views

9 Times Cited - Web of Science