Journal article
Expression of inositol 1,4,5-trisphosphate receptors changes the Ca2+ signal of Xenopus oocytes
American Journal of Physiology: Cell Physiology, Vol.270(4), pp.C1255-C1261
04/01/1996
DOI: 10.1152/ajpcell.1996.270.4.C1255
PMID: 8928753
Abstract
The receptors for the second messenger inositol 1,4,5-trisphosphate [Ins(1,4,5)P3] form a family of closely related proteins that play an important role in regulating the free intracellular Ca2+ concentration. To test the hypothesis that changing the expression level of Ins(1,4,5)P3 receptors could alter the Ins(1,4,5)P3-mediated Ca2+ signal, we overexpressed Ins(1,4,5)P3 receptor type 1 (InsP3R-1) or type 3 (InsP3R-3) in Xenopus laevis oocytes. Expression of InsP3R-1 increased the velocity of the propagating waves of intracellular Ca2+ release but did not affect the Ins(1,4,5)P3-induced entry of extracellular Ca2+ across the plasma membrane. In contrast, expression of intracellular Ca2+ but markedly increased the magnitude and duration of Ca2+ influx. Immunolocalization studied revealed InsP3R-3 at the endoplasmic reticulum, with a relatively stronger signal at or near the plasma membrane. The results suggest that changing the expression level of an InsP3R can alter the Ins(1,4,5)P3-mediated Ca2+ signal and that InsP3R-1 and InsP3R-3 may have different biological functions.
Details
- Title: Subtitle
- Expression of inositol 1,4,5-trisphosphate receptors changes the Ca2+ signal of Xenopus oocytes
- Creators
- Sylvain Delisle - Veterans Affairs Medical Center, Iowa City, Iowa, USAOlivier Blondel - Veterans Affairs Medical Center, Iowa City, Iowa, USAFrank J Longo - Veterans Affairs Medical Center, Iowa City, Iowa, USAWilliam E Schnabel - Veterans Affairs Medical Center, Iowa City, Iowa, USAGraeme I Bell - Veterans Affairs Medical Center, Iowa City, Iowa, USAMichael J Welsh - Veterans Affairs Medical Center, Iowa City, Iowa, USA
- Resource Type
- Journal article
- Publication Details
- American Journal of Physiology: Cell Physiology, Vol.270(4), pp.C1255-C1261
- DOI
- 10.1152/ajpcell.1996.270.4.C1255
- PMID
- 8928753
- ISSN
- 0363-6143
- eISSN
- 1522-1563
- Language
- English
- Date published
- 04/01/1996
- Academic Unit
- Neurology; Molecular Physiology and Biophysics; Pulmonary, Critical Care, and Occupational Medicine; Anatomy and Cell Biology; Fraternal Order of Eagles Diabetes Research Center; Neurosurgery; Internal Medicine
- Record Identifier
- 9984020862802771
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