Journal article
Expression of monocyte chemoattractant protein (MCP-1) and nitric oxide synthase-2 following cerebral trauma
Acta neuropathologica, Vol.95(1), pp.98-103
12/1997
DOI: 10.1007/s004010050770
PMID: 9452827
Abstract
Traumatic injury to the brain initiates multiple interrelated processes that involve parenchymal, vascular, and infiltrating inflammatory cells. Nitric oxide (NO) and chemokines have been implicated as regulators of the central nervous system injury response. Following a cryogenic lesion of the cerebral cortex in mice, mRNA for NO synthase (NOS)-2 was detected by reverse transcriptase polymerase chain reaction ipsilaterally 12 h after injury and persisted for 2 weeks. While mRNA was also detected contralaterally, the time course of expression was shorter (1 week). By immunohistochemistry, NOS-2 protein was initially detected ipsilaterally 12 h after injury in infiltrating inflammatory cells. Astroglial cells expressed NOS-2 from 24 to 72 h after injury. The expression of monocyte chemoattractant protein (MCP-1) mRNA peaked at 6 h on the lesion side, remained for 24 h and then declined by 48 h. On the unlesioned side, MCP-1 mRNA was expressed to a much lesser extent and had declined by 24 h. The up-regulation of MCP-1 was relatively specific as a closely related mRNA encoding IP-10 was not significantly increased. These findings implicate a role for NOS-2 and MCP-1 as potential regulators of cellular events following cryogenic cerebral trauma.
Details
- Title: Subtitle
- Expression of monocyte chemoattractant protein (MCP-1) and nitric oxide synthase-2 following cerebral trauma
- Creators
- Dana Grzybicki - Department of Pathology, University of Iowa College of Medicine, Iowa City, IA 52242, USA USSteven A Moore - Department of Pathology, University of Iowa College of Medicine, Iowa City, IA 52242, USA USRobert Schelper - Department of Pathology, University of Iowa College of Medicine, Iowa City, IA 52242, USA USAndrzej R Glabinski - Department of Neurosciences, Cleveland Clinic Foundation, Cleveland, OH 44195, USA USRichard M Ransohoff - Department of Neurosciences, Cleveland Clinic Foundation, Cleveland, OH 44195, USA USSean Murphy - Department of Pharmacology, BSB, University of Iowa College of Medicine, Iowa City, IA 52242, USA Tel.: 1-319-335-7958; Fax: 1-319-335-8930; e-mail: sean-murphy@uiowa.edu US
- Resource Type
- Journal article
- Publication Details
- Acta neuropathologica, Vol.95(1), pp.98-103
- Publisher
- Springer-Verlag; Berlin/Heidelberg
- DOI
- 10.1007/s004010050770
- PMID
- 9452827
- ISSN
- 0001-6322
- eISSN
- 1432-0533
- Language
- English
- Date published
- 12/1997
- Academic Unit
- Pathology
- Record Identifier
- 9984047714302771
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