Expression of protein F, the fibronectin-binding protein of Streptococcus pyogenes JRS4, in heterologous streptococcal and enterococcal strains promotes their adherence to respiratory epithelial cells

Emanuel Hanski; Phillip A Horwitz; Michael G Caparon

doi:10.1128/IAI.60.12.5119-5125.1992

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Expression of protein F, the fibronectin-binding protein of Streptococcus pyogenes JRS4, in heterologous streptococcal and enterococcal strains promotes their adherence to respiratory epithelial cells

Journal article

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Peer reviewed

Expression of protein F, the fibronectin-binding protein of Streptococcus pyogenes JRS4, in heterologous streptococcal and enterococcal strains promotes their adherence to respiratory epithelial cells

Emanuel Hanski, Phillip A Horwitz and Michael G Caparon

Infection and immunity, Vol.60(12), pp.5119-5125

12/1992

DOI: 10.1128/IAI.60.12.5119-5125.1992

PMCID: PMC258286

PMID: 1452345

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Abstract

In a previous study we reported the identification of protein F, a fibronectin-binding protein that was essential to the ability of Streptococcus pyogenes JRS4 to adhere to respiratory epithelial cells (E. Hanski and M. Caparon, Proc. Natl. Acad. Sci. USA, 89:6172-6176, 1992). To further evaluate the role of protein F in the adherence of the group A streptococci, we screened other group A streptococcal strains, including six recent clinical isolates, and one strain of Enterococcus faecalis for their capacity to bind fibronectin and for the presence of the gene encoding protein F (prtF). Seven of eight group A streptococcal strains analyzed, including all recent clinical isolates, both bound fibronectin at high affinity and contained DNA sequences that hybridized with a prtF-specific probe. One group A streptococcal isolate and the strain of E. faecalis examined neither contained a prtF-related gene nor bound fibronectin. These two strains also could not efficiently adhere to respiratory epithelial cells. However, upon the introduction of the cloned prtF gene, both of these strains gained the capacity to bind fibronectin and to adhere to respiratory epithelial cells. These results suggest that protein F is an important adhesin, which may have a general role in the virulence of the group A streptococci.

Cricetinae

Virulence

Bacterial Proteins - genetics

Cells, Cultured

Streptococcus pyogenes - physiology

Streptococcus pyogenes - metabolism

Fibronectins - metabolism

Bacterial Adhesion

Animals

Enterococcus faecalis - metabolism

Receptors, Fibronectin - genetics

Trachea - microbiology

Bacterial Proteins - biosynthesis

Receptors, Fibronectin - physiology

Details

Title: Subtitle: Expression of protein F, the fibronectin-binding protein of Streptococcus pyogenes JRS4, in heterologous streptococcal and enterococcal strains promotes their adherence to respiratory epithelial cells
Creators: Emanuel Hanski - Department of Molecular Microbiology, Washington University School of Medicine, St. Louis, Missouri 63110-1093
Phillip A Horwitz
Michael G Caparon
Resource Type: Journal article
Publication Details: Infection and immunity, Vol.60(12), pp.5119-5125
DOI: 10.1128/IAI.60.12.5119-5125.1992
PMID: 1452345
PMCID: PMC258286
ISSN: 0019-9567
eISSN: 1098-5522
Language: English
Date published: 12/1992
Academic Unit: Cardiovascular Medicine; Internal Medicine
Record Identifier: 9984094757102771

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