Expression of receptor protein tyrosine phosphatase alpha mRNA in human prostate cancer cell lines

Stanislav Zelivianski; Jeanenne Dean; Deepak Madhavan; Fen-Fen Lin; Ming-Fong Lin

doi:10.1023/A:1007010304194

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Expression of receptor protein tyrosine phosphatase alpha mRNA in human prostate cancer cell lines

Journal article

Peer reviewed

Expression of receptor protein tyrosine phosphatase alpha mRNA in human prostate cancer cell lines

Stanislav Zelivianski, Jeanenne Dean, Deepak Madhavan, Fen-Fen Lin and Ming-Fong Lin

Molecular and cellular biochemistry, Vol.208(1-2), pp.11-18

05/2000

DOI: 10.1023/A:1007010304194

PMID: 10939623

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Abstract

Receptor protein tyrosine phosphatase alpha (RPTPalpha) is a transmembrane protein phosphatase, and has been proposed to be involved in the differentiation of the neuronal system. In the present study, we demonstrated the expression of RPTPalpha mRNA in several normal human tissues. We further investigated the regulation of expression of RPTPalpha mRNA in epithelial cells utilizing three commercially available human prostate cancer cell lines LNCaP, PC-3 and DU145. This is because these cells exhibit different levels of differentiation, defined by the expression of a tissue-specific differentiation antigen, prostatic acid phosphatase (PAcP), and their androgen sensitivity. LNCaP cells express PAcP and are androgen-sensitive cells, while PC-3 and DU145 cells do not express PAcP and are androgen-insensitive cells. Northern blot analyses revealed that, in LNCaP cells, fetal bovine serum (FBS) and 5alpha-dihydrotestosterone (DHT) down-regulates RPTPalpha mRNA expression, similar to the effect on PAcP. Contrarily, FBS up-regulated the RPTPalpha mRNA level in PC-3 and DU145 cells. In LNCaP cells, sodium butyrate inhibited cell growth and up-regulated RPTPalpha as well as PAcP mRNA expression. Although, sodium butyrate also inhibited the growth of PC-3 and DU145 cells, the level of RPTPalpha mRNA was decreased in PC-3, while increased in DU145 cells. Thus, data taken together indicate that the expression of RPTPalpha is apparently regulated by a similar mechanism to that of PAcP in LNCaP cells.

Prostatic Neoplasms - metabolism

Tetradecanoylphorbol Acetate - pharmacology

Butyrates - pharmacology

Humans

Gene Expression Regulation, Neoplastic

Male

Protein Tyrosine Phosphatases - metabolism

Receptor-Like Protein Tyrosine Phosphatases, Class 4

RNA, Messenger - metabolism

Acid Phosphatase - metabolism

Protein Tyrosine Phosphatases - genetics

Carcinogens - pharmacology

Prostatic Neoplasms - genetics

Cattle

Dihydrotestosterone - pharmacology

Tumor Cells, Cultured

Serum Albumin, Bovine - pharmacology

RNA, Messenger - genetics

Receptors, Cell Surface - metabolism

Fetal Proteins - pharmacology

Blotting, Northern

Animals

Prostatic Neoplasms - enzymology

Acid Phosphatase - genetics

Receptors, Cell Surface - genetics

Details

Title: Subtitle: Expression of receptor protein tyrosine phosphatase alpha mRNA in human prostate cancer cell lines
Creators: Stanislav Zelivianski - Department of Biochemistry/Molecular Biology, University of Nebraska Medical Center, Omaha 68198-4525, USA
Jeanenne Dean
Deepak Madhavan
Fen-Fen Lin
Ming-Fong Lin
Resource Type: Journal article
Publication Details: Molecular and cellular biochemistry, Vol.208(1-2), pp.11-18
DOI: 10.1023/A:1007010304194
PMID: 10939623
ISSN: 0300-8177
eISSN: 1573-4919
Grant note: CA 72274 / NCI NIH HHS
Language: English
Date published: 05/2000
Academic Unit: General Internal Medicine; Internal Medicine
Record Identifier: 9984094657302771

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