Expression of select immune genes (surfactant proteins A and D, sheep beta defensin 1, and toll-like receptor 4) by respiratory epithelia is developmentally regulated in the preterm neonatal lamb

David K Meyerholz; Kenji Kawashima; Jack M Gallup; Branka Grubor; Mark R Ackermann

doi:10.1016/j.dci.2006.01.001

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Expression of select immune genes (surfactant proteins A and D, sheep beta defensin 1, and toll-like receptor 4) by respiratory epithelia is developmentally regulated in the preterm neonatal lamb

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Expression of select immune genes (surfactant proteins A and D, sheep beta defensin 1, and toll-like receptor 4) by respiratory epithelia is developmentally regulated in the preterm neonatal lamb

David K Meyerholz, Kenji Kawashima, Jack M Gallup, Branka Grubor and Mark R Ackermann

Developmental and comparative immunology, Vol.30(11), pp.1060-1069

2006

DOI: 10.1016/j.dci.2006.01.001

PMCID: PMC2791064

PMID: 16510184

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Abstract

Preterm infants experience enhanced susceptibility and severity to respiratory syncytial virus (RSV) infection. Terminal airway epithelium is an important site of RSV infection and the extent of local innate immune gene expression is poorly understood. In this study, expression of surfactant proteins A and D (SP-AD), sheep beta defensin 1 (SBD1), and toll-like receptor 4 (TLR4) mRNA were determined in whole lung homogenates from lambs. SP-AD and TLR4 mRNA expression increased ( p<0.05) from late gestation to term birth. In addition, gene expression of LCM-retrieved type II pneumocytes (CD208+), adjacent epithelium (CD208−) and bronchial epithelium demonstrated that bronchiole-alveolar junction epithelium (combined CD208+/−) had significant ( p<0.05) developmental increases in SP-AD, SBD1 and TLR4 mRNA, whereas CD208+ cells had statistically significant increases only with SP-A mRNA. Using immunofluorescence, SP-AD antigen distribution and intensity were also greater with developmental age. These studies show reduced SBD1, SP-AD, and TLR4 expression in the preterm lung and this may underlie enhanced RSV susceptibility.

Beta-defensin

Preterm infant

Lung

Surfactant protein

Sheep

Toll-like receptor

Antimicrobial peptide

Details

Title: Subtitle: Expression of select immune genes (surfactant proteins A and D, sheep beta defensin 1, and toll-like receptor 4) by respiratory epithelia is developmentally regulated in the preterm neonatal lamb
Creators: David K Meyerholz - Department of Veterinary Pathology, 2740 College of Veterinary Medicine, Iowa State University, Ames, IA 50011-1250, USA
Kenji Kawashima - Department of Veterinary Pathology, 2740 College of Veterinary Medicine, Iowa State University, Ames, IA 50011-1250, USA
Jack M Gallup - Department of Veterinary Pathology, 2740 College of Veterinary Medicine, Iowa State University, Ames, IA 50011-1250, USA
Branka Grubor - Department of Veterinary Pathology, 2740 College of Veterinary Medicine, Iowa State University, Ames, IA 50011-1250, USA
Mark R Ackermann - Department of Veterinary Pathology, 2740 College of Veterinary Medicine, Iowa State University, Ames, IA 50011-1250, USA
Resource Type: Journal article
Publication Details: Developmental and comparative immunology, Vol.30(11), pp.1060-1069
DOI: 10.1016/j.dci.2006.01.001
PMID: 16510184
PMCID: PMC2791064
NLM abbreviation: Dev Comp Immunol
ISSN: 0145-305X
eISSN: 1879-0089
Publisher: Elsevier Ltd
Language: English
Date published: 2006
Academic Unit: Pathology
Record Identifier: 9984083832402771

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