Expression of staphylococcal enterotoxin C1 in Escherichia coli

Gregory A Bohach; Patrick M Schlievert

doi:10.1128/IAI.55.2.428-432.1987

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Expression of staphylococcal enterotoxin C1 in Escherichia coli

Journal article

Open access

Peer reviewed

Expression of staphylococcal enterotoxin C1 in Escherichia coli

Gregory A Bohach and Patrick M Schlievert

Infection and immunity, Vol.55(2), pp.428-432

02/1987

DOI: 10.1128/IAI.55.2.428-432.1987

PMCID: PMC260345

PMID: 3542834

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Abstract

The structural gene encoding staphylococcal enterotoxin C1 was cloned into Escherichia coli and localized on a 1.5-kilobase HindIII-ClaI DNA fragment by subcloning. The toxin was partially purified from E. coli clones and shown to be immunologically identical to enterotoxin C1 from Staphylococcus aureus. The cloned toxin also had the same molecular weight (26,000) and charge heterogeneity as staphylococcus-derived enterotoxin. Toxins from both sources were equally biologically active.

Plasmids

Rabbits

Enterotoxins - genetics

Animals

Molecular Weight

Enterotoxins - toxicity

Escherichia coli - genetics

Cloning, Molecular

Mice

Details

Title: Subtitle: Expression of staphylococcal enterotoxin C1 in Escherichia coli
Creators: Gregory A Bohach
Patrick M Schlievert
Resource Type: Journal article
Publication Details: Infection and immunity, Vol.55(2), pp.428-432
Publisher: United States
DOI: 10.1128/IAI.55.2.428-432.1987
PMID: 3542834
PMCID: PMC260345
ISSN: 0019-9567
eISSN: 1098-5522
Language: English
Date published: 02/1987
Academic Unit: Microbiology and Immunology; Internal Medicine
Record Identifier: 9984001108902771

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