Expression of type II iodothyronine deiodinase marks the time that a tissue responds to thyroid hormone-induced metamorphosis in Xenopus laevis

Liquan Cai; Donald D Brown

doi:10.1016/j.ydbio.2003.10.005

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Expression of type II iodothyronine deiodinase marks the time that a tissue responds to thyroid hormone-induced metamorphosis in Xenopus laevis

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Expression of type II iodothyronine deiodinase marks the time that a tissue responds to thyroid hormone-induced metamorphosis in Xenopus laevis

Liquan Cai and Donald D Brown

Developmental biology, Vol.266(1), pp.87-95

2004

DOI: 10.1016/j.ydbio.2003.10.005

PMID: 14729480

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Abstract

The thyroid gland synthesizes thyroxine (T4), which passes through the larval tadpole's circulatory system. The enzyme type II iodothyronine deiodinase (D2) converts thyroxine (T4) to the active hormone 3,5,3′-triiodothyronine (T3) in peripheral tissues. An early response to thyroid hormone (TH) in the Xenopus laevis tadpole is the stimulation of cell division in cells that line the brain ventricles, the lumen of the spinal cord, and the limb buds. These cells express constitutively high levels of D2 mRNA. Exogenous T4 induces early DNA synthesis in brain, spinal cord, and limb buds as efficiently as T3. The deiodinase inhibitor iopanoic acid blocks T4- but not T3-induced cell division. At metamorphic climax, both TH-induced cell division and D2 expression decrease in the brain. Then D2 expression appears in late-responding tissues including the anterior pituitary, the intestine, and the tail where cell division is reduced or absent. Therefore, constitutive expression of D2 occurs in the earliest target tissues of TH that will grow and differentiate, while TH-induced expression of D2 takes place in late-responding tissues that will remodel or die. This pattern of constitutive and induced D2 expression contributes to the timing of metamorphic changes in these tissues.

Brain

Cell division

Deiodinase

Gene expression

Intestine

Metamorphosis

Spinal cord

Tail

Thyroid hormone

Xenopus laevis

Details

Title: Subtitle: Expression of type II iodothyronine deiodinase marks the time that a tissue responds to thyroid hormone-induced metamorphosis in Xenopus laevis
Creators: Liquan Cai - Carnegie Institution for Science
Donald D Brown - Carnegie Institution for Science
Resource Type: Journal article
Publication Details: Developmental biology, Vol.266(1), pp.87-95
DOI: 10.1016/j.ydbio.2003.10.005
PMID: 14729480
NLM abbreviation: Dev Biol
ISSN: 0012-1606
eISSN: 1095-564X
Publisher: Elsevier Inc
Language: English
Date published: 2004
Academic Unit: Cardiovascular Medicine; Internal Medicine
Record Identifier: 9984361574502771

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