Expression of xenobiotic metabolising enzymes in lungs of horses with or without histological evidence of lower airway inflammation

Maria Lofgren; Pia Larsson; Ronny Lindberg; Katarina Hornaeus; Eva Tyden

doi:10.1002/vms3.331

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Expression of xenobiotic metabolising enzymes in lungs of horses with or without histological evidence of lower airway inflammation

Journal article

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Expression of xenobiotic metabolising enzymes in lungs of horses with or without histological evidence of lower airway inflammation

Maria Lofgren, Pia Larsson, Ronny Lindberg, Katarina Hornaeus and Eva Tyden

Veterinary medicine and science, Vol.7(1), pp.16-24

01/2021

DOI: 10.1002/vms3.331

PMCID: PMC7840205

PMID: 32791560

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Abstract

Mild, moderate and severe equine asthma is a problem for equine welfare. The aetiology of the disease is not known in detail but is likely multi-factorial. One important factor may be inhaled dust which carries harmful substances which may be bioactivated and thus can lead to local inflammation in the airways. The aim of this study was to investigate gene expression and protein localisation of cytochrome P450 (CYP) enzymes, superoxide dismutase and glutathione-S-transferases (GST) involved in bioactivation and detoxification of harmful substances in lungs of horses with or without histological evidence of lower airway inflammation. Significantly lower gene expression of CYP2A13 and GSTM1 was observed in lungs from horses with histological evidence of lower airway inflammation compared with horses without. A higher expression, although not significant, was found for CYP1A1 in horses with histological evidence of lower airway inflammation. There were no differences in gene expression of GSTP1 and SOD3. The proteins were localised in the respiratory epithelium which is of relevance as a defence to local exposure of inhaled harmful substances. In conclusion, our study reports differential gene expression of enzymes involved in bioactivation and detoxification of foreign substances in the lungs of horses with histological evidence of lower airway inflammation compared with horses without.

Life Sciences & Biomedicine

Science & Technology

Veterinary Sciences

Details

Title: Subtitle: Expression of xenobiotic metabolising enzymes in lungs of horses with or without histological evidence of lower airway inflammation
Creators: Maria Lofgren - Swedish University of Agricultural Sciences
Pia Larsson - Swedish University of Agricultural Sciences
Ronny Lindberg - Swedish University of Agricultural Sciences
Katarina Hornaeus - Swedish University of Agricultural Sciences
Eva Tyden - Swedish University of Agricultural Sciences
Resource Type: Journal article
Publication Details: Veterinary medicine and science, Vol.7(1), pp.16-24
DOI: 10.1002/vms3.331
PMID: 32791560
PMCID: PMC7840205
NLM abbreviation: Vet Med Sci
ISSN: 2053-1095
eISSN: 2053-1095
Publisher: Wiley
Number of pages: 9
Grant note: Swedish-Norwegian Foundation for Equine Research
Language: English
Date published: 01/2021
Academic Unit: Nursing
Record Identifier: 9984370742802771

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