Journal article
Expression pattern and in situ localization of the mouse homologue of the human MYOC (GLC1A) gene in adult brain
Molecular Brain Research, Vol.68(1), pp.64-72
1999
DOI: 10.1016/S0169-328X(99)00085-6
PMID: 10320784
Abstract
The MYOC ( GLC1A) gene has recently been associated with both juvenile-onset primary open angle glaucoma (JOAG) and typical late-onset primary open angle glaucoma (POAG). As a result, much scrutiny has been focused on the pathology of these diseases. In order to better understand the pathophysiology of POAG, we have been developing a mouse model of the disease. As a step in this development, we have investigated the expression pattern of Myoc transcripts in embryonic and adult mouse tissue using Northern blot and in situ hybridization analyses. Myoc transcripts were found in high levels in adult eye, heart, brain, skeletal muscle and testis and to a lesser extent in lung and kidney. They were also present, albeit in very low amounts, during mouse embryogenesis. We present new evidence using in situ hybridization analysis that Myoc transcripts were present in widespread regions of the adult brain including the ependymal lining of the third and fourth ventricles, in the choroid plexus, the zonal layer of the junction of the inferior and superior colliculi, the neurons of the habenula, the piriform cortex, the median pre-optic nucleus of the hypothalamus, the olfactory tubercle, and in the inferior olive. In a functional sense, Myoc expression in the ependyma and choroid plexus, two regions of the brain involved in cerebrospinal fluid synthesis and resorption, parallels Myoc expression in the ciliary body and trabecular meshwork of the anterior segment of the eye where aqueous humor synthesis and outflow occur.
Details
- Title: Subtitle
- Expression pattern and in situ localization of the mouse homologue of the human MYOC (GLC1A) gene in adult brain
- Creators
- Ruth E Swiderski - Department of Pediatrics, University of Iowa, Room 440 EMRB, Iowa City, IA 52242, USALihua Ying - University of IowaMartin D Cassell - Department of Anatomy, University of Iowa, Iowa City, IA 52242, USAWallace L.M Alward - Department of Ophthalmology, University of Iowa, Iowa City, IA 52242, USAEdwin M Stone - Department of Ophthalmology, University of Iowa, Iowa City, IA 52242, USAVal C Sheffield - Department of Pediatrics, University of Iowa, Room 440 EMRB, Iowa City, IA 52242, USA
- Resource Type
- Journal article
- Publication Details
- Molecular Brain Research, Vol.68(1), pp.64-72
- DOI
- 10.1016/S0169-328X(99)00085-6
- PMID
- 10320784
- NLM abbreviation
- Brain Res Mol Brain Res
- ISSN
- 0169-328X
- eISSN
- 1872-6941
- Publisher
- Elsevier B.V
- Language
- English
- Date published
- 1999
- Academic Unit
- Anatomy and Cell Biology; Stead Family Department of Pediatrics; Iowa Neuroscience Institute; Medical Genetics and Genomics; Ophthalmology and Visual Sciences
- Record Identifier
- 9983980391402771
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