Journal article
Expression profile of nerve growth factor after muscle incision in the rat
Anesthesiology (Philadelphia), Vol.110(1), pp.140-149
01/2009
DOI: 10.1097/ALN.0b013e318190bc84
PMCID: PMC2727137
PMID: 19104181
Abstract
Previous studies have demonstrated that nerve growth factor (NGF) is an important mediator of pathologic pain. Many studies have focused on cutaneous mechanisms for NGF-induced hyperalgesia; few have examined its contribution in deeper tissues like muscle. This study examined pain behaviors and the expression of NGF in incised hind paw flexor digitorum brevis muscle.
Adult Sprague-Dawley rats were pretreated with anti-NGF peptibody and underwent skin or skin plus deep fascia and muscle incision. Guarding pain behaviors were measured. Muscle NGF messenger RNA (mRNA) was measured by reverse-transcriptase polymerase chain reaction. Changes in NGF protein expression were measured using Western blot, enzyme-linked immunosorbent assay, and immunohistochemistry. In situ hybridization for NGF mRNA was also performed.
Pretreatment with anti-NGF peptibody (100 mg/kg) decreased the guarding behavior caused by deep fascia and muscle incision. Muscle NGF mRNA increased abruptly 2 h after incision and was the same as control by postoperative day 1. NGF protein increased from 4 h after incision and was sustained for several days. NGF was localized in many calcitonin gene-related peptide-positive axons, few N52-positive axons, but not isolectin B4-positive axons in incised muscle. The sources of NGF mRNA included keratinocytes in epidermis and fibroblasts in deeper tissues.
Fibroblasts adjacent to the injury are sources of NGF in incised muscle. NGF is upregulated by incision of muscle and contributes to guarding pain behavior.
Details
- Title: Subtitle
- Expression profile of nerve growth factor after muscle incision in the rat
- Creators
- Chaoran Wu - University of IowaMark A Erickson - Roy J. and Lucille A. Carver College of MedicineJun Xu - University of IowaKenneth D Wild - AmgenTimothy J Brennan - University of Iowa
- Resource Type
- Journal article
- Publication Details
- Anesthesiology (Philadelphia), Vol.110(1), pp.140-149
- DOI
- 10.1097/ALN.0b013e318190bc84
- PMID
- 19104181
- PMCID
- PMC2727137
- ISSN
- 0003-3022
- eISSN
- 1528-1175
- Grant note
- R29 GM055831-05 / NIGMS NIH HHS F32 GM067752 / NIGMS NIH HHS GM-067752 / NIGMS NIH HHS R29 GM055831 / NIGMS NIH HHS GM-55831 / NIGMS NIH HHS
- Language
- English
- Date published
- 01/2009
- Academic Unit
- Surgery; Anesthesia
- Record Identifier
- 9984322936202771
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