Expression profiling of transcription factors Pax-5, Oct-1, Oct-2, BOB.1, and PU.1 in Hodgkin's and non-Hodgkin's lymphomas: a comparative study using high throughput tissue microarrays

Ryan C McCune; Sergei I Syrbu; Mohammad A Vasef

doi:10.1038/modpathol.3800622

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Expression profiling of transcription factors Pax-5, Oct-1, Oct-2, BOB.1, and PU.1 in Hodgkin's and non-Hodgkin's lymphomas: a comparative study using high throughput tissue microarrays

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Expression profiling of transcription factors Pax-5, Oct-1, Oct-2, BOB.1, and PU.1 in Hodgkin's and non-Hodgkin's lymphomas: a comparative study using high throughput tissue microarrays

Ryan C McCune, Sergei I Syrbu and Mohammad A Vasef

Modern pathology, Vol.19(7), pp.1010-1018

07/2006

DOI: 10.1038/modpathol.3800622

PMID: 16648862

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Abstract

Analysis of B-cell-specific transcription factors is useful in understanding of the differentiation-linked phenotype in Hodgkin's as well as in non-Hodgkin's lymphomas. We analyzed the expression profiling of transcription factors Pax-5, Oct-1, Oct-2, BOB.1, and PU.1 in 109 cases, including non-Hodgkin's lymphomas of B- and T-lineage, classical Hodgkin's lymphomas, and nodular lymphocyte predominant Hodgkin's lymphomas. Our study revealed that all transcription factors were universally expressed in all cases of nodular lymphocyte predominant Hodgkin's and variably expressed in non-Hodgkin's lymphomas of B-lineage. Cases of classical Hodgkin's lymphoma variably expressed the Pax-5, Oct-1, Oct-2, and BOB.1. However, in contrast to nodular lymphocyte predominant Hodgkin's lymphoma, the transcription factor PU.1 was consistently absent in all cases of classical Hodgkin's lymphoma. Transcription factors Pax-5, BOB.1, and PU.1 were not detectable in cases of anaplastic large cell lymphoma. However, the Oct-1 was detected in all anaplastic large cells lymphoma cases, indicating that expression of this transcription factor was not restricted to B-lineage lymphoid malignancies. Our findings suggest that inclusion of the PU.1 antibody may prove useful in separating classical Hodgkin's lymphomas from nodular lymphocyte predominant Hodgkin's lymphomas in problematic cases.

Immunohistochemistry

Proto-Oncogene Proteins - metabolism

Diagnosis, Differential

Hodgkin Disease - metabolism

Hodgkin Disease - pathology

Tissue Array Analysis

Humans

Lymphoma, Non-Hodgkin - pathology

Octamer Transcription Factor-2 - metabolism

Lymphoma, Non-Hodgkin - metabolism

Trans-Activators - metabolism

Retrospective Studies

Octamer Transcription Factor-1 - metabolism

PAX5 Transcription Factor - metabolism

Details

Title: Subtitle: Expression profiling of transcription factors Pax-5, Oct-1, Oct-2, BOB.1, and PU.1 in Hodgkin's and non-Hodgkin's lymphomas: a comparative study using high throughput tissue microarrays
Creators: Ryan C McCune - Department of Pathology, University of Iowa Hospital and Clinics, Iowa City, IA, USA
Sergei I Syrbu
Mohammad A Vasef
Resource Type: Journal article
Publication Details: Modern pathology, Vol.19(7), pp.1010-1018
Publisher: United States
DOI: 10.1038/modpathol.3800622
PMID: 16648862
ISSN: 0893-3952
eISSN: 1530-0285
Language: English
Date published: 07/2006
Academic Unit: Pathology
Record Identifier: 9984046825302771

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