Journal article
Expression, purification and characterization of recombinant human microsomal PGE2 synthase-1
Advances in experimental medicine and biology, Vol.525, pp.113-116
01/01/2003
DOI: 10.1007/978-1-4419-9194-2_22
PMID: 12751747
Abstract
Prostaglandin E synthases (PGES) comprise a number of structurally different enzymes that convert the product of the cyclooxygenase enzymes, PGH2, into the physiologically important PGE2. An inducible microsomal PGES (mPGES-1) couples preferentially with COX-2 [1], whereas a constitutively and widely expressed cytosolic PGES (cPGES or p23) is believed to couple with Cox-1 [2]. Both of these enzyme activities are dependent on GSH. Most recently, a second, membrane-associated PGES (mPGES-2) that has a wide tissue distribution and is activated by a number of thio1-containing reagents was identified and purified [3]. Two cytosolic glutathione transferases (μ2 and μ3) also have some PGES activity. As the expression of mPGES-1 is strongly induced by pro-inflammatory stimuli and is down-regulated by glucocorticoids, mPGES-1 is a potential drug target for anti-inflammatory therapy. A solubilization and partial purification of a microsomal PGES activity from bovine vesicular glands has been reported [4]. It appears that this enzyme is the same as mPGES-1. Here we report the baculovirus over-expression of recombinant human mPGES-1 in Sf9 insect cells, its purification to apparent homogeneity and the characterization of its enzymatic properties.
Details
- Title: Subtitle
- Expression, purification and characterization of recombinant human microsomal PGE2 synthase-1
- Creators
- Marc Ouellet - Merck-Frosst, CanadaAlly Pen - Merck-Frosst, CanadaPo-Hien Ear - MerckJean-Pierre Falgueyret - Merck-Frosst, CanadaTammy G LeRiche - Merck-Frosst, CanadaJoseph A Mancini - Merck-Frosst, CanadaDenis Riendeau - Merck-Frosst, CanadaM David Percival - Merck-Frosst, Canada
- Resource Type
- Journal article
- Publication Details
- Advances in experimental medicine and biology, Vol.525, pp.113-116
- DOI
- 10.1007/978-1-4419-9194-2_22
- PMID
- 12751747
- eISBN
- 9781441991942; 1441991948
- ISSN
- 0065-2598
- Language
- English
- Date published
- 01/01/2003
- Academic Unit
- Surgery
- Record Identifier
- 9984322954502771
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