Journal article
Extensive cytocidal replication of lactate dehydrogenase-elevating virus in cultured peritoneal macrophages from 1–2-week-old mice
Virus research, Vol.14(4), pp.327-338
1989
DOI: 10.1016/0168-1702(89)90025-7
PMID: 2623946
Abstract
Indirect fluorescent antibody staining was used to examine the replication of lactate dehydrogenase-elevating virus (LDV) in primary cultures of peritoneal macrophages from BALB/c mice of different ages. Up to 80% of the total peritoneal macrophages from 1–2-week-old mice were susceptible to productive infection by LDV, though only 1–2% of the cells expressed detectable levels of IA antigen. The proportion of LDV-permissive peritoneal macrophages progressively decreased to 5–15% between 2 and 5 weeks of age of the mice. Macrophages from 9-day-old mice, when cultured in the presence of L cell conditioned medium, retained undiminished LDV permissiveness for at least 10 days in culture. The maximum proportion of LDV antigen-positive cells was detected between 8–10 h post infection of macrophages cultured from both 1–2-week-old and adult mice, concomitant with maximum LDV RNA synthesis. The LDV antigen positive macrophages disappeared between 12 and 48 h post infection. In cultures of macrophages from 9–10-day old mice, the loss of infected cells was clearly due to cell killing, proving unequivocally that LDV replication is cytocidal. Disintegration of LDV-infected macrophages or phagocytosis of killed macrophages by surviving macrophages must be very sudden and complete since infected cells disappeared without the appearance of trypan blue-stainable cells in the culture. Ten cell lines established from macrophages of 2, 9, and 10-day-old mice all contained a small proportion of LDV-permissive cells (1–4%). Individual clones of one of the lines contained a similar small proportion of LDV-permissive cells.
Details
- Title: Subtitle
- Extensive cytocidal replication of lactate dehydrogenase-elevating virus in cultured peritoneal macrophages from 1–2-week-old mice
- Creators
- Chike O OnyekabaJohn T HartyPeter G.W Plagemann
- Resource Type
- Journal article
- Publication Details
- Virus research, Vol.14(4), pp.327-338
- Publisher
- Elsevier B.V
- DOI
- 10.1016/0168-1702(89)90025-7
- PMID
- 2623946
- ISSN
- 0168-1702
- eISSN
- 1872-7492
- Language
- English
- Date published
- 1989
- Academic Unit
- Pathology
- Record Identifier
- 9984046901402771
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