Journal article
Extracellular Vesicle-Mediated siRNA Delivery, Protein Delivery, and CFTR Complementation in Well-Differentiated Human Airway Epithelial Cells
Genes, Vol.11(4), p.351
04/01/2020
DOI: 10.3390/genes11040351
PMCID: PMC7230663
PMID: 32224868
Abstract
Extracellular vesicles (EVs) are a class of naturally occurring secreted cellular bodies that are involved in long distance cell-to-cell communication. Proteins, lipids, mRNA, and miRNA can be packaged into these vesicles and released from the cell. This information is then delivered to target cells. Since EVs are naturally adapted molecular messengers, they have emerged as an innovative, inexpensive, and robust method to deliver therapeutic cargo in vitro and in vivo. Well-differentiated primary cultures of human airway epithelial cells (HAE) are refractory to standard transfection techniques. Indeed, common strategies used to overexpress or knockdown gene expression in immortalized cell lines simply have no detectable effect in HAE. Here we use EVs to efficiently deliver siRNA or protein to HAE. Furthermore, EVs can deliver CFTR protein to cystic fibrosis donor cells and functionally correct the Cl- channel defect in vitro. EV-mediated delivery of siRNA or proteins to HAE provides a powerful genetic tool in a model system that closely recapitulates the in vivo airways.
Details
- Title: Subtitle
- Extracellular Vesicle-Mediated siRNA Delivery, Protein Delivery, and CFTR Complementation in Well-Differentiated Human Airway Epithelial Cells
- Creators
- Brajesh K. Singh - Roy J. and Lucille A. Carver College of MedicineAshley L. Cooney - Roy J. and Lucille A. Carver College of MedicineSateesh Krishnamurthy - Roy J. and Lucille A. Carver College of MedicinePatrick L. Sinn - Roy J. and Lucille A. Carver College of Medicine
- Resource Type
- Journal article
- Publication Details
- Genes, Vol.11(4), p.351
- DOI
- 10.3390/genes11040351
- PMID
- 32224868
- PMCID
- PMC7230663
- NLM abbreviation
- Genes (Basel)
- ISSN
- 2073-4425
- eISSN
- 2073-4425
- Publisher
- Mdpi
- Number of pages
- 15
- Grant note
- SINN15XX1; SINN15XX0; SINN19XX0 / Cystic Fibrosis Foundation; Italian Cystic Fibrosis Research Foundation NIH R01 HL-133089; NIH R01 AI-132402 / National Institutes of Health; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA
- Language
- English
- Date published
- 04/01/2020
- Academic Unit
- Microbiology and Immunology; Pulmonary Medicine; Stead Family Department of Pediatrics
- Record Identifier
- 9984297431402771
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