Extracellular redox state regulates features associated with prostate cancer cell invasion

Luksana Chaiswing; Weixiong Zhong; Joseph J Cullen; Larry W Oberley; Terry D Oberley

doi:10.1158/0008-5472.CAN-08-0162

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Extracellular redox state regulates features associated with prostate cancer cell invasion

Journal article

Open access

Peer reviewed

Extracellular redox state regulates features associated with prostate cancer cell invasion

Luksana Chaiswing, Weixiong Zhong, Joseph J Cullen, Larry W Oberley and Terry D Oberley

Cancer research (Chicago, Ill.), Vol.68(14), pp.5820-5826

07/15/2008

DOI: 10.1158/0008-5472.CAN-08-0162

PMID: 18632636

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Abstract

We have examined the possible role of extracellular reduction-oxidation (redox) state in regulation of biological/biochemical features associated with prostate cancer cell invasion. DU145, PC-3, and RWPE1-derived human prostate cancer (WPE1-NB26) cell lines were used for the present in vitro analysis. Increasing levels of nitric oxide using S-nitroso-N-acetylpenicillamine resulted in a decrease in cell invasion ability, whereas increasing levels of extracellular superoxide radical (O(2)(*-)) using xanthine/xanthine oxidase resulted in an increase in cell invasion ability in these three cell lines. WPE1-NB26 cells exhibited an increased glutathione/glutathione disulfide ratio in the medium in comparison with RWPE1 cells (immortalized but nonmalignant prostate epithelial cells), suggesting an alteration of extracellular redox state of WPE1-NB26 cells. We hypothesized that O(2)(*-) production at or near the plasma membrane or in the adjacent extracellular matrix at least partially regulated prostate cancer cell invasion. Using adenovirus-mediated extracellular superoxide dismutase (EC-SOD) gene transduction to enzymatically decrease O(2)(*-) levels, we showed that in the presence of heparin, adenovirus EC-SOD gene transduction resulted in an increase in the expression of EC-SOD outside the cells with resultant inhibition of cell invasion ability. This inhibition correlated with reduced metalloproteinase [matrix metalloproteinase (MMP) 2/membrane type 1-MMP] activities and increased levels of extracellular nitrite. Our results suggest a prominent role of extracellular redox status in regulation of cell invasion, which may provide opportunities for therapeutic interventions.

Prostatic Neoplasms - pathology

Models, Chemical

Oxidation-Reduction

Glutathione - metabolism

Neoplasm Invasiveness

Humans

Gene Expression Regulation, Neoplastic

NADPH Oxidases - metabolism

Nitrites - metabolism

Male

Oxygen - metabolism

Models, Biological

Cell Line, Tumor

Matrix Metalloproteinases - metabolism

Superoxide Dismutase - metabolism

Details

Title: Subtitle: Extracellular redox state regulates features associated with prostate cancer cell invasion
Creators: Luksana Chaiswing - Pathology and Laboratory Medicine Service, William S. Middleton Memorial Veterans Hospital, Department of Pathology and Laboratory Medicine, School of Medicine and Public Health, University of Wisconsin, Madison, Wisconsin 53705, USA
Weixiong Zhong
Joseph J Cullen
Larry W Oberley
Terry D Oberley
Resource Type: Journal article
Publication Details: Cancer research (Chicago, Ill.), Vol.68(14), pp.5820-5826
DOI: 10.1158/0008-5472.CAN-08-0162
PMID: 18632636
NLM abbreviation: Cancer Res
ISSN: 0008-5472
eISSN: 1538-7445
Publisher: United States
Language: English
Date published: 07/15/2008
Academic Unit: Surgery; Radiation Oncology
Record Identifier: 9984051507002771

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