Journal article
FGF2 gene activated matrices promote proliferation of bone marrow stromal cells
Archives of oral biology, Vol.60(12), pp.1742-1749
12/2015
DOI: 10.1016/j.archoralbio.2015.09.005
PMCID: PMC4636935
PMID: 26433191
Abstract
•Functional transfection of PEI-pDNA (FGF-2) in BMSCs was demonstrated.•FGF-2 gene activated matrix promoted attachment and proliferation of BMSCs.•This strategy has immense clinical translational potential. In this study, we report on the results from the development and early in vitro testing of a gene activated matrix encoding basic human fibroblast growth factor 2 (FGF2) in bone marrow stromal cells (BMSCs). Polyethylenimine (PEI), a cationic polymer, was utilized as a gene delivery vector and collagen scaffolds were used as the carrier to deliver the PEI-pDNA nano-sized complexes (nanoplexes) encoding the FGF2 protein. Initially, the BMSCs were transfected in vitro with the PEI-pFGF2 nanoplexes, prepared at a N/P ratio of 10, with cells alone and naked DNA as controls. This was followed by transfection experiments using collagen scaffold containing complexes, with the scaffold alone as a control. The transfection efficacy of the nanoplexes was assessed using ELISA for the determination of FGF2 protein expressed by the transfected cells. The functionality of transfection was assessed by evaluating cellular recruitment, attachment, and proliferation of BMSCs on the scaffold using imaging techniques. BMSCs transfected with the PEI-pFGF2 nanoplexes (either alone or within the scaffold) led to higher expression of FGF2, compared to controls. Scanning electron microscopy and confocal imaging confirmed the recruitment and attachment of BMSCs to scaffolds containing the PEI-pFGF2 nanoplexes. Confocal microscopy showed a significantly higher number of proliferating cells within PEI-pFGF2 nanoplex-loaded scaffolds than with empty scaffolds. This first in vitro evaluation in BMSCs provides evidence that gene activated matrices (GAMs) encoding the FGF2 protein may have strong translational potential for clinical applications that require enhanced osseous and periodontal tissue regeneration.
Details
- Title: Subtitle
- FGF2 gene activated matrices promote proliferation of bone marrow stromal cells
- Creators
- Sheetal D’Mello - Division of Pharmaceutics and Translational Therapeutics, College of Pharmacy, University of Iowa, IA, USASatheesh Elangovan - Department of Periodontics, College of Dentistry, University of Iowa, IA, USAAliasger K Salem - Division of Pharmaceutics and Translational Therapeutics, College of Pharmacy, University of Iowa, IA, USA
- Resource Type
- Journal article
- Publication Details
- Archives of oral biology, Vol.60(12), pp.1742-1749
- DOI
- 10.1016/j.archoralbio.2015.09.005
- PMID
- 26433191
- PMCID
- PMC4636935
- NLM abbreviation
- Arch Oral Biol
- ISSN
- 0003-9969
- eISSN
- 1879-1506
- Publisher
- Elsevier Ltd
- Language
- English
- Date published
- 12/2015
- Academic Unit
- Roy J. Carver Department of Biomedical Engineering; Pharmaceutical Sciences and Experimental Therapeutics; Craniofacial Anomalies Research Center; Dental Research; Chemical and Biochemical Engineering; Periodontics
- Record Identifier
- 9983985714502771
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