Journal article
FK506 rescues peripheral nerve allografts in acute rejection
Journal of neurotrauma, Vol.18(2), pp.217-229
02/01/2001
DOI: 10.1089/08977150150502631
PMID: 11229713
Abstract
This study investigated the ability of the immunosuppressant FK506 to reverse nerve allograft rejection in progress. Eighty-four Buffalo rats received posterior tibial nerve grafts from either Lewis or Buffalo donor animals. Allografts were left untreated for either 7, 10, or 14 days before receiving daily subcutaneous FK506 injections (2 mg/kg). Time-matched control animals received either an isograft, an allograft with continuous FK506, or an allograft with no postoperative FK506 therapy. All animals underwent weekly evaluation of nerve function by walking track analysis. Experimental group animals were sacrificed either immediately prior to initiation of FK506 therapy (days 7, 10, or 14), after 2 weeks of immunosuppressive treatment, or 8 weeks postsurgery. Histomorphometric analysis, consisting of measurements of total number of nerve fibers, neural density, and percent of neural debris, demonstrated a statistically significant increase in regeneration in the isograft group relative to the untreated allograft group within 28 days of transplantation. Grafts harvested from animals receiving 2 weeks of FK506 after 7 or 10 days of rejection were histomorphometrically similar to time-matched isografts. By contrast, grafts from animals receiving 2 weeks of FK506 following 14 days without therapy resembled untreated allografts and demonstrated significant histomorphometric differences from isografts at the corresponding time point. Analysis of walking track data confirmed that relative to untreated allografts, functional recovery was hastened in animals receiving an isograft, or allograft treated with FK506. This study demonstrated that when started within 10 days of graft placement, FK506 could reverse nerve allograft rejection in rats evaluated following 2 weeks of treatment.
Details
- Title: Subtitle
- FK506 rescues peripheral nerve allografts in acute rejection
- Creators
- Felix Y Feng - Washington University in St. LouisM. Allison Ogden - Division of Plastic and Reconstructive Surgery, Washington University School of Medicine, St. Louis, Missouri, United StatesTerence M Myckatyn - Washington University in St. LouisAaron G Grand - Washington University in St. LouisJohn N Jensen - Washington University in St. LouisDaniel A Hunter - Washington University in St. LouisSusan E Mackinnon - Washington University in St. Louis
- Resource Type
- Journal article
- Publication Details
- Journal of neurotrauma, Vol.18(2), pp.217-229
- Publisher
- Liebert
- DOI
- 10.1089/08977150150502631
- PMID
- 11229713
- ISSN
- 0897-7151
- eISSN
- 1557-9042
- Number of pages
- 13
- Language
- English
- Date published
- 02/01/2001
- Academic Unit
- Surgery
- Record Identifier
- 9984701552502771
Metrics
4 Record Views