Journal article
FOXL2 and SOX9 Distinguish the Lineage of the Sex Cord–Stromal Cells in Gonadoblastomas
Pediatric and developmental pathology, Vol.14(5), pp.391-395
09/2011
DOI: 10.2350/10-12-0943-OA.1
PMID: 21682576
Abstract
Gonadoblastomas are mixed germ cell sex cord–stromal tumors that arise in dysgenetic gonads and are composed of immature germ cells and sex cord–stromal cells of indeterminate differentiation. FOXL2 is one of the first genes expressed in female gonad development, and it is required for proper granulosa cell differentiation during folliculogenesis. SOX9,adownstream target of SRY, the gene in the Y chromosomal sex-determining region, is required for testicular development and for the formation and maintenance of (pre-)Sertoli cells. This study characterized the sex cord–stromal cells of gonadoblastoma by evaluating the expression of these counteracting transcription factors. Archival paraffin-embedded material of 7 gonadoblastomas, 5 of which were overgrown by dysgerminoma, was examined by immunohistochemistry for expression and localization of FOXL2 and SOX9. The sex cord–stromal cells revealed strong nuclear staining for FOXL2 and were negative for SOX9 expression. Germ cells in the gonadoblastoma and dysgerminoma components showed no FOXL2 and SOX9 expression. Areas of transition between gonadoblastoma and dysgerminoma revealed nests with a gradual reduction of FOXL2 expression. Our results support the hypothesis that the sex cord–stromal cell component of gonadoblastomas is of granulosa cell origin. In addition, FOXL2 appears to be a useful marker for the evaluation of overgrowth by dysgerminomas and for the identification of the transition zone of “dysgerminoma in situ.” As FOXL2 and SOX9 are differentially expressed, they also should be useful for distinguishing gonadoblastomas from intratubular germ cell neoplasias and can help to differentiate those with a Sertoli cell component from gonadoblastoma with a granulosa cell component.
Details
- Title: Subtitle
- FOXL2 and SOX9 Distinguish the Lineage of the Sex Cord–Stromal Cells in Gonadoblastomas
- Creators
- Rebecca Buell-Gutbrod - Department of Pathology, University of Chicago, Chicago, IL, USAMarina Ivanovic - Department of Pathology, University of Chicago, Chicago, IL, USAAnthony Montag - Department of Pathology, University of Chicago, Chicago, IL, USAErnst Lengyel - Department of Gynecology, University of Chicago, Chicago, IL, USAOluwole Fadare - Department of Pathology, Vanderbilt University, Nashville, TN, USAKatja Gwin - Department of Pathology, University of Chicago, Chicago, IL, USA
- Resource Type
- Journal article
- Publication Details
- Pediatric and developmental pathology, Vol.14(5), pp.391-395
- DOI
- 10.2350/10-12-0943-OA.1
- PMID
- 21682576
- ISSN
- 1093-5266
- eISSN
- 1615-5742
- Language
- English
- Date published
- 09/2011
- Academic Unit
- Pathology
- Record Identifier
- 9984047791402771
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