FPRP, a major, highly stoichiometric, highly specific CD81- and CD9-associated protein

Christopher S Stipp; David Orlicky; Martin E Hemler

doi:10.1074/jbc.M009859200

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FPRP, a major, highly stoichiometric, highly specific CD81- and CD9-associated protein

Journal article

Open access

Peer reviewed

FPRP, a major, highly stoichiometric, highly specific CD81- and CD9-associated protein

Christopher S Stipp, David Orlicky and Martin E Hemler

The Journal of biological chemistry, Vol.276(7), pp.4853-4862

02/16/2001

DOI: 10.1074/jbc.M009859200

PMID: 11087758

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Abstract

CD81 and CD9, members of the transmembrane-4 superfamily (TM4SF; tetraspanins), form extensive complexes with other TM4SF proteins, integrins, and other proteins, especially in mild detergents. In moderately stringent Brij 96 lysis conditions, CD81 and CD9 complexes are virtually identical to each other, but clearly distinct from other TM4SF complexes. One of the most prominent proteins within CD81 and CD9 complexes is identified here as FPRP, the 133-kDa prostaglandin F(2alpha) receptor regulatory protein. FPRP, a cell-surface Ig superfamily protein, associates specifically with CD81 or with CD81 and CD9, but not with integrins or other TM4SF proteins. In contrast to other CD81- and CD9-associating proteins, FPRP associates at very high stoichiometry, with essentially 100% of cell-surface FPRP on 293 cells being CD81- and CD9-associated. Also, CD81.CD9.FPRP complexes have a discrete size (<4 x 10(6) Da) as measured by gel permeation chromatography and remain intact after disruption of cholesterol-rich membrane microdomains by methyl-beta-cyclodextrin. Although CD81 associated with both alpha(3) integrin and FPRP in 293 cells, the alpha(3)beta(1).CD81 and CD81.CD9.FPRP complexes were distinct, as determined by immunoprecipitation and immunodepletion experiments. In conclusion, our data affirm the existence of distinct TM4SF complexes with unique compositions and specifically characterize FPRP as the most robust, highly stoichiometric CD81- and/or CD9-associated protein yet described.

Membrane Microdomains - metabolism

Polyethylene Glycols - chemistry

Tetraspanin 28

Antigens, CD - metabolism

Macromolecular Substances

Membrane Proteins

Animals

Proteins - metabolism

Neoplasm Proteins

Plant Oils

Membrane Glycoproteins

Tumor Cells, Cultured

Tetraspanin-29

Details

Title: Subtitle: FPRP, a major, highly stoichiometric, highly specific CD81- and CD9-associated protein
Creators: Christopher S Stipp - Dana-Farber Cancer Institute and the Department of Pathology, Harvard Medical School, 44 Binney Street, Boston, MA 02115, USA
David Orlicky
Martin E Hemler
Resource Type: Journal article
Publication Details: The Journal of biological chemistry, Vol.276(7), pp.4853-4862
DOI: 10.1074/jbc.M009859200
PMID: 11087758
NLM abbreviation: J Biol Chem
ISSN: 0021-9258
eISSN: 1083-351X
Publisher: United States
Grant note: GM38903 / NIGMS NIH HHS
Language: English
Date published: 02/16/2001
Academic Unit: Molecular Physiology and Biophysics; Biology
Record Identifier: 9983991999402771

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