FSH receptor (FSHR) expression in human extragonadal reproductive tissues and the developing placenta, and the impact of its deletion on pregnancy in mice

Julie A W Stilley; Debora E Christensen; Kristin B Dahlem; Rongbin Guan; Donna A Santillan; Sarah K England; Ayman Al-Hendy; Patricia A Kirby; Deborah L Segaloff

doi:10.1095/biolreprod.114.118562

Back

FSH receptor (FSHR) expression in human extragonadal reproductive tissues and the developing placenta, and the impact of its deletion on pregnancy in mice

Journal article

Open access

Peer reviewed

FSH receptor (FSHR) expression in human extragonadal reproductive tissues and the developing placenta, and the impact of its deletion on pregnancy in mice

Julie A W Stilley, Debora E Christensen, Kristin B Dahlem, Rongbin Guan, Donna A Santillan, Sarah K England, Ayman Al-Hendy, Patricia A Kirby and Deborah L Segaloff

Biology of reproduction, Vol.91(3), pp.74-74

09/2014

DOI: 10.1095/biolreprod.114.118562

PMCID: PMC4435062

PMID: 25100706

Files and links (1)

url

https://doi.org/10.1095/biolreprod.114.118562View

Published (Version of record) Open Access

Abstract

Expression and function of the follicle-stimulating hormone receptor (FSHR) in females were long thought to be limited to the ovary. Here, however, we identify extragonadal FSHR in both the human female reproductive tract and the placenta, and test its physiological relevance in mice. We show that in nonpregnant women FSHR is present on: endothelial cells of blood vessels in the endometrium, myometrium, and cervix; endometrial glands of the proliferative and secretory endometrium; cervical glands and the cervical stroma; and (at low levels) stromal cells and muscle fibers of the myometrium. In pregnant women, placental FSHR was detected as early as 8-10 wk of gestation and continued through term. It was expressed on: endothelial cells in fetal portions of the placenta and the umbilical cord; epithelial cells of the amnion; decidualized cells surrounding the maternal arteries in the maternal decidua; and the stromal cells and muscle fibers of the myometrium, with particularly strong expression at term. These findings suggest that FSHR expression is upregulated during decidualization and upregulated in myometrium as a function of pregnancy. The presence of FSHR in the placental vasculature suggests a role in placental angiogenesis. Analysis of genetically modified mice in which Fshr is lacking in fetal portions of the placenta revealed adverse effects on fetoplacental development. Our data further demonstrate FSHB and CGA mRNAs in placenta and uterus, consistent with potential local sources of FSH. Collectively, our data suggest heretofore unappreciated roles of extragonadal FSHR in female reproductive physiology.

Endothelium, Vascular - cytology

Immunohistochemistry

Cervix Uteri - blood supply

Up-Regulation

Cervix Uteri - metabolism

Receptors, FSH - genetics

Humans

Umbilical Cord - cytology

RNA, Messenger - metabolism

Umbilical Cord - metabolism

Gene Expression Regulation, Developmental

Placenta - cytology

Myometrium - metabolism

Adult

Female

Endometrium - cytology

Endometrium - metabolism

Receptors, FSH - metabolism

Myometrium - blood supply

Placentation

Stromal Cells - metabolism

Extraembryonic Membranes - cytology

Mice, Knockout

Myometrium - cytology

Placenta - metabolism

Pregnancy

Placenta - blood supply

Animals

Endothelium, Vascular - metabolism

Extraembryonic Membranes - blood supply

Extraembryonic Membranes - metabolism

Cervix Uteri - cytology

Stromal Cells - cytology

Endometrium - blood supply

Details

Title: Subtitle: FSH receptor (FSHR) expression in human extragonadal reproductive tissues and the developing placenta, and the impact of its deletion on pregnancy in mice
Creators: Julie A W Stilley - Department of Molecular Biophysics and Physiology, University of Iowa Roy J. and Lucille A. Carver College of Medicine, Iowa City, Iowa
Debora E Christensen - Department of Molecular Biophysics and Physiology, University of Iowa Roy J. and Lucille A. Carver College of Medicine, Iowa City, Iowa
Kristin B Dahlem - Department of Molecular Biophysics and Physiology, University of Iowa Roy J. and Lucille A. Carver College of Medicine, Iowa City, Iowa
Rongbin Guan - Department of Molecular Biophysics and Physiology, University of Iowa Roy J. and Lucille A. Carver College of Medicine, Iowa City, Iowa
Donna A Santillan - Department of Obstetrics and Gynecology, University of Iowa Roy J. and Lucille A. Carver College of Medicine, Iowa City, Iowa
Sarah K England - Department of Obstetrics and Gynecology, Washington University School of Medicine, St. Louis, Missouri
Ayman Al-Hendy - Department of Obstetrics and Gynecology, Meharry Medical Center, Nashville, Tennessee
Patricia A Kirby - Department of Pathology, University of Iowa Roy J. and Lucille A. Carver College of Medicine, Iowa City, Iowa
Deborah L Segaloff - Department of Molecular Biophysics and Physiology, University of Iowa Roy J. and Lucille A. Carver College of Medicine, Iowa City, Iowa deborah-segaloff@uiowa.edu
Resource Type: Journal article
Publication Details: Biology of reproduction, Vol.91(3), pp.74-74
DOI: 10.1095/biolreprod.114.118562
PMID: 25100706
PMCID: PMC4435062
ISSN: 0006-3363
eISSN: 1529-7268
Grant note: R01 HD046228 / NICHD NIH HHS R01 HD022196 / NICHD NIH HHS T32DK007690, / NIDDK NIH HHS HD022196 / NICHD NIH HHS T32 DK007690 / NIDDK NIH HHS R37 HD022196 / NICHD NIH HHS HD037831 / NICHD NIH HHS R01 HD037831 / NICHD NIH HHS
Language: English
Date published: 09/2014
Academic Unit: Molecular Physiology and Biophysics; Pathology; Obstetrics and Gynecology; Medicine Administration
Record Identifier: 9984083227602771

Metrics

20 Record Views

93 Times Cited - Web of Science

See more details