Journal article
FXYD3 facilitates Na 1 and liquid absorption across human airway epithelia by increasing the transport capacity of the Na/K ATPase
American Journal of Physiology: Cell Physiology, Vol.323(4), pp.C1044-C1051
10/01/2022
DOI: 10.1152/ajpcell.00047.2022
PMCID: PMC9529271
PMID: 35993520
Abstract
Na/K ATPase activity is essential for ion transport across epithelia. FXYD3, a y subunit of the Na/K ATPase, is expressed in the airway, but its function remains undetermined. Single-cell RNA sequencing and immunohistochemistry revealed that FXYD3 localizes within the basolateral membrane of all airway epithelial cells. To study FXYD3 function, we reduced FXYD3 expression using siRNA. After permeabilizing the apical membrane with nystatin, epithelia pretreated with FXYD3-targeting siRNA had lower ouabain-sensitive short-circuit currents than control epithelia. FXYD3-targeting siRNA also reduced amiloride-sensitive short-cir-cuit currents and liquid absorption across intact epithelia. These data are consistent with FXYD3 facilitating Na+ and liquid absorption. FXYD3 may be needed to maintain the high rates of Na + and fluid absorption observed for airway and other FXYD3-expressing epithelia.
Details
- Title: Subtitle
- FXYD3 facilitates Na 1 and liquid absorption across human airway epithelia by increasing the transport capacity of the Na/K ATPase
- Creators
- Camilo Cano Portillo - Roy J. and Lucille A. Carver College of MedicineRaul Villacreses - University of IowaAndrew L. Thurman - University of IowaAlejandro A. Pezzulo - University of IowaJoseph Zabner - University of IowaIan M. Thornell - Roy J. and Lucille A. Carver College of Medicine
- Resource Type
- Journal article
- Publication Details
- American Journal of Physiology: Cell Physiology, Vol.323(4), pp.C1044-C1051
- DOI
- 10.1152/ajpcell.00047.2022
- PMID
- 35993520
- PMCID
- PMC9529271
- NLM abbreviation
- Am J Physiol Cell Physiol
- ISSN
- 0363-6143
- eISSN
- 1522-1563
- Publisher
- Amer Physiological Soc
- Number of pages
- 8
- Grant note
- Cystic Fibrosis Foundation (Iowa Research Development Program) National Institutes of Health?s National Heart, Lung, and Blood Institute; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Heart Lung & Blood Institute (NHLBI) Gilead Sciences Research Scholars Program in Cystic Fibrosis
- Language
- English
- Date published
- 10/01/2022
- Academic Unit
- Pulmonary, Critical Care, and Occupational Medicine; Iowa Neuroscience Institute; Internal Medicine
- Record Identifier
- 9984359564802771
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