Journal article
Fabrication and in vitro testing of polymeric delivery system for condensed DNA
Journal of biomedical materials research, Vol.67A(4), pp.1384-1392
2003
DOI: 10.1002/jbm.a.20036
PMID: 14624526
Abstract
Polyethylenimine (PEI) was combined with plasmid DNA and freeze dried following the addition of sucrose as a lyoprotectant and pore-forming agent. Freeze-dried PEI DNA condensates were dry mixed with granular polylactideglycolic acid (PLGA) then compression molded and sponged to encapsulated PEI DNA. A measurement of the elastic modulus indicated that 91 wt% sucrose substituted for 95 wt% sodium chloride as a porogen, resulting in PLGA sponges with a mechanical modulus of 100 kPa. The PEI DNA was retained (80%) within PLGA sponges prepared with sucrose during the leaching and subsequent 2-week release studies, whereas sodium chloride PLGA sponges caused the premature release (100%) of PEI DNA within 2 days. In vitro gene transfer studies with PEI DNA PLGA sponges established that adherent and infiltrating fibroblasts expressed reporter gene for 15 days compared with the short, 3-day expression mediated by direct gene of PEI DNA on cells in culture. The results demonstrate an approach to encapsulate condensed DNA in a PLGA sponge for the purpose of retaining DNA within the matrices and creating efficient gene transfer during tissue engineering.
Details
- Title: Subtitle
- Fabrication and in vitro testing of polymeric delivery system for condensed DNA
- Creators
- Yen-Chen HUANG - College of Pharmacy, University of Michigan, Ann Arbor, Michigan 48109-1065, United StatesMaureen CONNELL - College of Pharmacy, University of Michigan, Ann Arbor, Michigan 48109-1065, United StatesYoumie PARK - College of Pharmacy, University of Michigan, Ann Arbor, Michigan 48109-1065, United StatesDavid J MOONEY - Department of Biomedical Engineering, University of Michigan, Ann Arbor, Michigan 48109-1065, United StatesKevin G RICE - College of Pharmacy, University of Michigan, Ann Arbor, Michigan 48109-1065, United States
- Resource Type
- Journal article
- Publication Details
- Journal of biomedical materials research, Vol.67A(4), pp.1384-1392
- DOI
- 10.1002/jbm.a.20036
- PMID
- 14624526
- NLM abbreviation
- J Biomed Mater Res
- ISSN
- 0021-9304
- eISSN
- 1097-4636
- Publisher
- John Wiley & Sons; New York, NY
- Language
- English
- Date published
- 2003
- Academic Unit
- Pharmaceutical Sciences and Experimental Therapeutics; Craniofacial Anomalies Research Center; Nephrology; Medicinal and Natural Products Chemistry; Internal Medicine
- Record Identifier
- 9984065313402771
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