Factors affecting the efficiency of embryo transfer in the domestic ferret ( Mustela putorius furo)

Ziyi Li; Xingshen Sun; Juan Chen; Gregory H Leno; John F Engelhardt

doi:10.1016/j.theriogenology.2005.10.021

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Factors affecting the efficiency of embryo transfer in the domestic ferret ( Mustela putorius furo)

Journal article

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Factors affecting the efficiency of embryo transfer in the domestic ferret ( Mustela putorius furo)

Ziyi Li, Xingshen Sun, Juan Chen, Gregory H Leno and John F Engelhardt

Theriogenology, Vol.66(2), pp.183-190

2006

DOI: 10.1016/j.theriogenology.2005.10.021

PMCID: PMC1893552

PMID: 16330092

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https://doi.org/10.1016/j.theriogenology.2005.10.021View

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Abstract

Embryo transfer (ET) to recipient females is a foundational strategy for a number of assisted reproductive technologies, including cloning by somatic cell nuclear transfer. In an attempt to develop efficient ET in domestic ferrets, factors affecting development of transferred embryo were investigated. Unilateral and bilateral transfer of zygotes or blastocysts in the oviduct or uterus was evaluated in recipient nulliparous or primiparous females. Developing fetuses were collected from recipient animals 21 days post-copulation and examined. The percentage of fetal formation was different ( P < 0.05) for unilateral and bilateral transfer of zygotes (71%) in nulliparous females with bilateral transfer (56%) in primiparous recipients. The percentage (90%) of fetal formation in nulliparous recipients following unilateral transfer of blastocysts was higher ( P < 0.05) than that observed in primiparous recipients with bilateral ET (73%). Notably, the percentage of fetal formation was higher ( P < 0.05) when blastocyts were transferred as compared to zygotes (90% versus 71%). Transuterine migration of embryos occurred following all unilateral transfers and also in approximately 50% of bilateral transfers with different number of embryos in each uterine horn. These data will help to facilitate the development of assisted reproductive strategies in the ferret and could lead to the use of this species for modeling human disease and for conservation of the endangered Mustelidae species such as black-footed ferret and European mink.

Embryo transfer

Nulliparous female

Embryo development

Domestic ferret

Primiparous female

Details

Title: Subtitle: Factors affecting the efficiency of embryo transfer in the domestic ferret ( Mustela putorius furo)
Creators: Ziyi Li - Department of Anatomy and Cell Biology, College of Medicine, University of Iowa, Iowa City, IA, USA
Xingshen Sun - Department of Anatomy and Cell Biology, College of Medicine, University of Iowa, Iowa City, IA, USA
Juan Chen - Department of Anatomy and Cell Biology, College of Medicine, University of Iowa, Iowa City, IA, USA
Gregory H Leno - Department of Anatomy and Cell Biology, College of Medicine, University of Iowa, Iowa City, IA, USA
John F Engelhardt - Department of Anatomy and Cell Biology, College of Medicine, University of Iowa, Iowa City, IA, USA
Resource Type: Journal article
Publication Details: Theriogenology, Vol.66(2), pp.183-190
DOI: 10.1016/j.theriogenology.2005.10.021
PMID: 16330092
PMCID: PMC1893552
NLM abbreviation: Theriogenology
ISSN: 0093-691X
eISSN: 1879-3231
Publisher: Elsevier Inc
Language: English
Date published: 2006
Academic Unit: Roy J. Carver Department of Biomedical Engineering; Anatomy and Cell Biology; Radiation Oncology; Internal Medicine
Record Identifier: 9984025309402771

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